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Literature summary extracted from

  • Fossati, E.; Polentini, F.; Carrea, G.; Riva, S.
    Exploitation of the alcohol dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid (2006), Biotechnol. Bioeng., 93, 1216-1220.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.2 analysis the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Levilactobacillus brevis
1.1.1.2 analysis the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Thermoanaerobacter brockii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.2 additional information evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Levilactobacillus brevis
1.1.1.2 additional information evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Thermoanaerobacter brockii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 12-ketochenodeoxycholic acid inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially Levilactobacillus brevis
1.1.1.2 acetone at high concentrations of e.g. 3.4 M Levilactobacillus brevis
1.1.1.2 acetone at high concentrations of e.g. 3.4 M Thermoanaerobacter brockii
1.1.1.2 cholic acid inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially Levilactobacillus brevis
1.1.1.176 acetone at 25% v/v and above Clostridium sp.
1.1.1.176 additional information no substrate inhibition at high concentration of cholic acid Clostridium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.2 2-propanol + NADP+ Levilactobacillus brevis
-
 acetone + NADPH
-
 r
1.1.1.2 2-propanol + NADP+ Thermoanaerobacter brockii
-
 acetone + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Levilactobacillus brevis
-
-
-
1.1.1.2 Thermoanaerobacter brockii
-
-
-
1.1.1.176 Clostridium sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.2 commercial preparation
-
 Levilactobacillus brevis
-
1.1.1.2 commercial preparation
-
 Thermoanaerobacter brockii
-
1.1.1.176 commercial preparation enzyme in 50% glycerol-containing solution Clostridium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.176 additional information
-
 assay method development using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, optimization, overview Clostridium sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.2 1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, 51% and 46% residual activity, respectively Levilactobacillus brevis
1.1.1.2 1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, quite stable Thermoanaerobacter brockii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 2-propanol + NADP+
-
 Levilactobacillus brevis acetone + NADPH
-
 r
1.1.1.2 2-propanol + NADP+
-
 Thermoanaerobacter brockii acetone + NADPH
-
 r
1.1.1.176 cholic acid + NADP+ the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview Clostridium sp. 12-ketochenodeoxycholic acid + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 ADH
-
 Levilactobacillus brevis
1.1.1.2 ADH
-
 Thermoanaerobacter brockii
1.1.1.176 12alpha-HSDH
-
 Clostridium sp.
1.1.1.176 NADP-dependent 12alpha-hydroxysteroid dehydrogenase
-
 Clostridium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 22
-
 assay at room temperature Levilactobacillus brevis
1.1.1.2 22
-
 assay at room temperature Thermoanaerobacter brockii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 8
-
 assay at Levilactobacillus brevis
1.1.1.2 8
-
 assay at Thermoanaerobacter brockii
1.1.1.176 8
-
-
 Clostridium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADP+ dependent on Levilactobacillus brevis
1.1.1.2 NADP+ dependent on Thermoanaerobacter brockii
1.1.1.2 NADPH dependent on Levilactobacillus brevis
1.1.1.2 NADPH dependent on Thermoanaerobacter brockii
1.1.1.176 NADP+
-
 Clostridium sp.