Literature summary extracted from

Youshko, M.I.; Moody, H.M.; Bukhanov, A.L.; Boosten, W.H.; Svedas, V.K.
Penicillin acylase-catalyzed synthesis of beta-lactam antibiotics in highly condensed aqueous systems: beneficial impact of kinetic substrate supersaturation (2004), Biotechnol. Bioeng., 85, 323-329.

Application

EC Number	Application	Comment	Organism
3.5.1.11	synthesis	enzyme-catalyzed synthesis of beta-lactam antibiotics in supersaturated solutions of substrates. Due to nucleophile supersaturation, ratio of synthesis/hydrolysis be be increased up to threefold	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.11	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.11	amoxicillin + H2O	-	Escherichia coli	D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid	-	r
3.5.1.11	ampicillin + H2O	-	Escherichia coli	(R)-2-phenylglycine + 6-aminopenicillanic acid	-	r
3.5.1.11	cephalexin + H2O	-	Escherichia coli	D-phenylglycine + 7-aminodesacetoxycephalosporanic acid	-	r
3.5.1.11	D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid	-	Escherichia coli	amoxicillin + NH3	-	r
3.5.1.11	D-phenylglycine amide + 6-aminopenicillanic acid	-	Escherichia coli	ampicillin + NH3	-	r
3.5.1.11	D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid	-	Escherichia coli	cephalexin + NH3	-	r

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Release 2023.1 (January 2023)