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Literature summary extracted from

  • Kim, J.S.; Koh, S.; Shin, H.J.; Lee, D.S.; Lee, S.Y.
    Biochemical characterization of a UDP-sugar pyrophosphorylase from Thermus caldophilus GK24 (1999), Biotechnol. Appl. Biochem., 29, 11-17.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.64 fructose 1-phosphate 2 mM, 78% activation Thermus caldophilus
2.7.7.64 galactose 6-phosphate 2 mM, 70% activation Thermus caldophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 0.2
-
 UTP pH 8.0, 73°C Thermus caldophilus
2.7.7.64 0.6
-
 N-acetylglucosamine 1-phosphate pH 8.0, 73°C Thermus caldophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.64 Co2+ divalent metal required. Mn2+ (3 mM) is 63% as effective as Mg2+ (3 mM) Thermus caldophilus
2.7.7.64 Mg2+ divalent metal required. Order of decreasing effectiveness: Mg2+ > Mn2+ > Co2+. Optimal concentration of Mg2+ is 5 mM Thermus caldophilus
2.7.7.64 Mn2+ divalent metal required. Mn2+ (3 mM) is 87% as effective as Mg2+ (3 mM) Thermus caldophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.64 41000
-
 1 * 41000, SDS-PAGE Thermus caldophilus
2.7.7.64 45000
-
 gel filtration Thermus caldophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Thermus caldophilus
-
 GK24
-
2.7.7.64 Thermus caldophilus GK24
-
 GK24
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.64
-
 Thermus caldophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 UTP + D-galactose 1-phosphate 3% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus diphosphate + UDP-galactose
-
 ?
2.7.7.64 UTP + D-galactose 1-phosphate 3% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus GK24 diphosphate + UDP-galactose
-
 ?
2.7.7.64 UTP + D-glucose 1-phosphate 159% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus diphosphate + UDP-glucose
-
 ?
2.7.7.64 UTP + D-glucose 1-phosphate 159% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus GK24 diphosphate + UDP-glucose
-
 ?
2.7.7.64 UTP + D-mannose 1-phosphate 5% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus diphosphate + UDP-mannose
-
 ?
2.7.7.64 UTP + D-mannose 1-phosphate 5% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus GK24 diphosphate + UDP-mannose
-
 ?
2.7.7.64 UTP + D-xylose 1-phosphate 44% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus diphosphate + UDP-alpha-D-xylopyranose
-
 ?
2.7.7.64 UTP + D-xylose 1-phosphate 44% of the activity with N-acetylglucosamine 1-phosphate Thermus caldophilus GK24 diphosphate + UDP-alpha-D-xylopyranose
-
 ?
2.7.7.64 UTP + N-acetylglucosamine 1-phosphate
-
 Thermus caldophilus diphosphate + UDP-N-acetylglucosamine
-
 ?
2.7.7.64 UTP + N-acetylglucosamine 1-phosphate
-
 Thermus caldophilus GK24 diphosphate + UDP-N-acetylglucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.64 monomer 1 * 41000, SDS-PAGE Thermus caldophilus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 UDP-sugar pyrophosphorylase
-
 Thermus caldophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.64 86
-
-
 Thermus caldophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.64 65 95 65°C: about 60% of maximal activity, 95°C: 90% of maximal activity Thermus caldophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.64 95
-
 half-life: 30 min Thermus caldophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.64 0.2
-
 N-acetylglucosamine 1-phosphate pH 8.0, 73°C Thermus caldophilus
2.7.7.64 0.4
-
 UTP pH 8.0, 73°C Thermus caldophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.64 6.9
-
 in presence of Mg2+ Thermus caldophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.64 5.5 8 pH 5.5: about 90% of maximal activity, pH 8.0: about 75% of maximal activity Thermus caldophilus