BRENDA - Enzyme Database

Partial purification and some properties of a phospholipase C from Pseudomonas sp. strain KS3.2

Sugimori, D.; Nakamura, M.; Biosci. Biotechnol. Biochem. 70, 535-537 (2006)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
2-mercaptoethanol
2 mM, 40% inhibition
Pseudomonas sp.
3.1.4.3
CoCl2
2 mM, 90% inhibition
Pseudomonas sp.
3.1.4.3
CuSO3
2 mM, 96% inhibition
Pseudomonas sp.
3.1.4.3
dithiothreitol
2 mM, 94% inhibition
Pseudomonas sp.
3.1.4.3
EDTA
2 mM, 92% inhibition
Pseudomonas sp.
3.1.4.3
FeCl2
2 mM, 94% inhibition
Pseudomonas sp.
3.1.4.3
FeCl3
2 mM, 90% inhibition
Pseudomonas sp.
3.1.4.3
HgCl2
2 mM, 92% inhibition
Pseudomonas sp.
3.1.4.3
MnCl2
2 mM, 92% inhibition
Pseudomonas sp.
3.1.4.3
phenylmethylsulfonyl fluoride
2 mM, 94% inhibition
Pseudomonas sp.
3.1.4.3
SDS
2 mM, 90% inhibition
Pseudomonas sp.
3.1.4.3
ZnCl2
2 mM, 89% inhibition
Pseudomonas sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas sp.
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
additional information
enzyme belongs to the group of metallophospholipases C
Pseudomonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Pseudomonas sp.
-
-
-
3.1.4.3
Pseudomonas sp. KS3.2
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
partial
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
additional information
phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine
668022
Pseudomonas sp.
?
-
-
-
-
3.1.4.3
additional information
phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine
668022
Pseudomonas sp. KS3.2
?
-
-
-
-
3.1.4.3
phosphatidic acid + H2O
9.04% of the activity with phosphatidylcholine
668022
Pseudomonas sp.
?
-
-
-
?
3.1.4.3
phosphatidic acid + H2O
9.04% of the activity with phosphatidylcholine
668022
Pseudomonas sp. KS3.2
?
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
668022
Pseudomonas sp.
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
668022
Pseudomonas sp. KS3.2
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.3
50
-
-
Pseudomonas sp.
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
3.1.4.3
35
60
35C: about 50% of maximal activity, 60C: about 50% of maximal activity
Pseudomonas sp.
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.3
52
-
pH 7.2, 30 min, 50% loss of activity
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7.2
-
-
Pseudomonas sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.3
6.5
8.5
pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity
Pseudomonas sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.3
1
2
5C, 3 h, about 55% loss of activity
Pseudomonas sp.
3.1.4.3
6
-
5C, 3 h, about 70% loss of activity
Pseudomonas sp.
3.1.4.3
7
-
5C, 3 h, about 35% loss of activity
Pseudomonas sp.
3.1.4.3
8
9
5C, 3 h, stable
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
2-mercaptoethanol
2 mM, 40% inhibition
Pseudomonas sp.
3.1.4.3
CoCl2
2 mM, 90% inhibition
Pseudomonas sp.
3.1.4.3
CuSO3
2 mM, 96% inhibition
Pseudomonas sp.
3.1.4.3
dithiothreitol
2 mM, 94% inhibition
Pseudomonas sp.
3.1.4.3
EDTA
2 mM, 92% inhibition
Pseudomonas sp.
3.1.4.3
FeCl2
2 mM, 94% inhibition
Pseudomonas sp.
3.1.4.3
FeCl3
2 mM, 90% inhibition
Pseudomonas sp.
3.1.4.3
HgCl2
2 mM, 92% inhibition
Pseudomonas sp.
3.1.4.3
MnCl2
2 mM, 92% inhibition
Pseudomonas sp.
3.1.4.3
phenylmethylsulfonyl fluoride
2 mM, 94% inhibition
Pseudomonas sp.
3.1.4.3
SDS
2 mM, 90% inhibition
Pseudomonas sp.
3.1.4.3
ZnCl2
2 mM, 89% inhibition
Pseudomonas sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Pseudomonas sp.
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
additional information
enzyme belongs to the group of metallophospholipases C
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
partial
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
additional information
phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine
668022
Pseudomonas sp.
?
-
-
-
-
3.1.4.3
additional information
phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine
668022
Pseudomonas sp. KS3.2
?
-
-
-
-
3.1.4.3
phosphatidic acid + H2O
9.04% of the activity with phosphatidylcholine
668022
Pseudomonas sp.
?
-
-
-
?
3.1.4.3
phosphatidic acid + H2O
9.04% of the activity with phosphatidylcholine
668022
Pseudomonas sp. KS3.2
?
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
668022
Pseudomonas sp.
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
668022
Pseudomonas sp. KS3.2
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.3
50
-
-
Pseudomonas sp.
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
3.1.4.3
35
60
35C: about 50% of maximal activity, 60C: about 50% of maximal activity
Pseudomonas sp.
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.3
52
-
pH 7.2, 30 min, 50% loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
7.2
-
-
Pseudomonas sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.3
6.5
8.5
pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity
Pseudomonas sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.3
1
2
5C, 3 h, about 55% loss of activity
Pseudomonas sp.
3.1.4.3
6
-
5C, 3 h, about 70% loss of activity
Pseudomonas sp.
3.1.4.3
7
-
5C, 3 h, about 35% loss of activity
Pseudomonas sp.
3.1.4.3
8
9
5C, 3 h, stable
Pseudomonas sp.