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Literature summary extracted from

  • Koga, Y.; Ohga, M.; Tsujimura, M.; Morii, H.; Kawarabayasi, Y.
    Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 7 (2006), Biosci. Biotechnol. Biochem., 70, 282-285.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.261 expressed in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.261 0.067
-
 NADPH at 65°C, pH 6.5, in 50 mM MOPS buffer Sulfurisphaera tokodaii
1.1.1.261 0.47
-
 dihydroxyacetone phosphate at 65°C, pH 6.5, in 50 mM MOPS buffer Sulfurisphaera tokodaii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.261 35000
-
 SDS-PAGE Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.261 Sulfurisphaera tokodaii
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.261 dihydroxyacetone phosphate + NAD(P)H
-
 Sulfurisphaera tokodaii sn-glycerol-1-phosphate + NAD(P)+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.261 G-1-P-dehydrogenase
-
 Sulfurisphaera tokodaii
1.1.1.261 sn-glycerol-1-phosphate dehydrogenase
-
 Sulfurisphaera tokodaii
1.1.1.261 ST0344 protein
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.261 80
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.261 37 85 at temperatures higher than 85°C no activity of the enzyme can be detected due to precipitation Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.261 6.2
-
 in MES buffer or MOPS buffer Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.261 5.6 7.7
-
 Sulfurisphaera tokodaii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.261 6.8
-
 at pH 6.8 86-91% of activity is detected Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.261 NAD(P)H
-
 Sulfurisphaera tokodaii