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Literature summary extracted from
- Purification and properties of NADP-dependent shikimate dehydrogenase from Gluconobacter oxydans IFO 3244 and its application to enzymatic shikimate production (2006), Biosci. Biotechnol. Biochem., 70, 2786-2789.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | 30000 | - |
1 * 30000, native PAGE | Gluconobacter oxydans |
| 1.1.1.25 | 30000 | - |
2 * 30000, native PAGE | Gluconobacter oxydans |
| 1.1.1.25 | 60000 | - |
gel filtration and native PAGE | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.25 | shikimate + NADP+ | Gluconobacter oxydans | - |
3-dehydroshikimate + NADPH + H+ | - |
r |  |
| 1.1.1.25 | shikimate + NADP+ | Gluconobacter oxydans IFO 3244 | - |
3-dehydroshikimate + NADPH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.25 | Gluconobacter oxydans | - |
- |
- |
| 1.1.1.25 | Gluconobacter oxydans IFO 3244 | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.25 | culture condition:quinate-grown cell | - |
Gluconobacter oxydans | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.25 | shikimate + NADP+ | - |
Gluconobacter oxydans | 3-dehydroshikimate + NADPH + H+ | - |
r | |
| 1.1.1.25 | shikimate + NADP+ | - |
Gluconobacter oxydans IFO 3244 | 3-dehydroshikimate + NADPH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.25 | dimer | 2 * 30000, native PAGE | Gluconobacter oxydans |
| 1.1.1.25 | monomer | 1 * 30000, native PAGE | Gluconobacter oxydans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.25 | NADP-dependent shikimate dehydrogenase | - |
Gluconobacter oxydans |
| 1.1.1.25 | SKDH | - |
Gluconobacter oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | 7 | - |
reduction of dehydroshikimate | Gluconobacter oxydans |
| 1.1.1.25 | 10 | - |
oxidation of shikimate | Gluconobacter oxydans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.25 | NADP+ | - |
Gluconobacter oxydans | |
| 1.1.1.25 | NADPH | - |
Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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