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Literature summary extracted from

  • Ohta, Y.; Nogi, Y.; Miyazaki, M.; Li, Z.; Hatada, Y.; Ito, S.; Horikoshi, K.
    Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from the novel marine isolate, JAMB-A94 (2004), Biosci. Biotechnol. Biochem., 68, 1073-1081.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 expression in Bacillus subtilis ISW 1214 Microbulbifer sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 1-ethyl-(3-(3-dimethyl-amino-)propyl)carbonate 1 mM. 10-20% inhibition Microbulbifer sp.
3.2.1.81 Cu2+ 1 mM Microbulbifer sp.
3.2.1.81 diethyl dicarbonate 1 mM, 10-20% inhibition Microbulbifer sp.
3.2.1.81 Hg2+ 1 mM Microbulbifer sp.
3.2.1.81 additional information not inhibited by iodoacetamide, p-chloromercuribenzoate, dithiothreitol, 2-mercaptoethanol (each at 1 mM), EDTA (100 mM) and 30 mM SDS Microbulbifer sp.
3.2.1.81 N-bromosuccinimide 0.1 mM Microbulbifer sp.
3.2.1.81 Pb2+ 1 mM Microbulbifer sp.
3.2.1.81 Zn2+ 1 mM Microbulbifer sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 32000
-
 SDS-PAGE Microbulbifer sp.
3.2.1.81 48200
-
 calculated from sequence Microbulbifer sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Microbulbifer sp.
-
-
-
3.2.1.81 Microbulbifer sp. JAMB-A94
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.81 proteolytic modification digestion during excretion Microbulbifer sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81 isolated from the sediment in Suruga bay, Japan, at a depth of 2406m, purification by ammonium sulfate precipitation, DEAE-Toyopearl 650M, gel filtration Microbulbifer sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.81 additional information extracellular, using Bacillus subtilis as the host Microbulbifer sp.
-
3.2.1.81 additional information isolated from the sediment in Suruga bay, Japan, at a depth of 2406m Microbulbifer sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 517.3
-
-
 Microbulbifer sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
 Microbulbifer sp. neoagarotetraose + neoagarohexaose + neoagarobiose
-
 ?
3.2.1.81 agarose + H2O
-
 Microbulbifer sp. JAMB-A94 neoagarotetraose + neoagarohexaose + neoagarobiose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 AgaA
-
 Microbulbifer sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 55
-
 15 min in 20 mM Tris-HCl buffer, pH 7.0 Microbulbifer sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 65
-
 retains 80% of original activity after 25 min Microbulbifer sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7
-
 Britton-Robinson universal buffers, 50°C Microbulbifer sp.