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Literature summary extracted from

  • Liu, S.L.; Wei, D.Z.; Song, Q.X.; Zhang, Y.W.; Wang, X.D.
    Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila (2006), Bioprocess Biosyst. Eng., 28, 285-289.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis enantioselective hydrolysis of N-phenylacetyl-DL-tert-leucine to yield L-tert-leucine. Rate of enzyme-catalyzed reaction is significantly affected by the presence of 2% organic cosolvent. Initial rate falls with increasing logP of the cosolvent, but for logP values less than -0.24, the rate is faster than that in aqueous medium. Yield of L-tert-leucine is above 95% with the enantiomeric excess of more than 99% Kluyvera cryocrescens

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.11 1,2-ethanediol 2% v/v, 154% of reaction rate in aqueous environment Kluyvera cryocrescens
3.5.1.11 dimethyl sulfoxide 2% v/v, 138% of reaction rate in aqueous environment Kluyvera cryocrescens
3.5.1.11 dioxane 2% v/v, 117% of reaction rate in aqueous environment Kluyvera cryocrescens
3.5.1.11 Glycerol 2% v/v, 179% of reaction rate in aqueous environment Kluyvera cryocrescens
3.5.1.11 Methanol 2% v/v, 126% of reaction rate in aqueous environment Kluyvera cryocrescens
3.5.1.11 N,N-dimethylformamide 2% v/v, 136% of reaction rate in aqueous environment Kluyvera cryocrescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Kluyvera cryocrescens
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 N-phenylacetyl-DL-tert-leucine + H2O enantioselective hydrolysis Kluyvera cryocrescens L-tert-leucine + phenylacetate
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