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Literature summary extracted from

  • Liu, B.; Bartlam, M.; Gao, R.; Zhou, W.; Pang, H.; Liu, Y.; Feng, Y.; Rao, Z.
    Crystal structure of the hyperthermophilic inorganic pyrophosphatase from the archaeon Pyrococcus horikoshii (2004), Biophys. J., 86, 420-427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expression in Escherichia coli strain BL21 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.1 purified recombinant enzyme, 20 mg/ml protien hanging drop vapour diffusion method, 3,8% PEG 4000, 0.1 M Na-acetate, pH 5.0-5.2, and 0.02 M MgCl2, asymmetric trimeric crystals, dimerization to hexamers, X-ray diffraction structure determination and analysis at 2.66 A resolution Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Pyrococcus horikoshii
-
2 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 recombinant enzyme from Escherichia coli strain BL21 Pyrococcus horikoshii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.1 diphosphate + H2O = 2 phosphate active site structure Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
Pyrococcus horikoshii 2 phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 hexamer dimeric trimer, crystal structure Pyrococcus horikoshii
3.6.1.1 More structural basis for the high thermostability Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 hyperthermophilic inorganic pyrophosphatase
-
Pyrococcus horikoshii
3.6.1.1 inorganic pyrophosphatase
-
Pyrococcus horikoshii
3.6.1.1 Pho-PPase
-
Pyrococcus horikoshii
3.6.1.1 PPase
-
Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 additional information
-
optimal growth at 88°C Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 additional information
-
structural basis for the high thermostability Pyrococcus horikoshii