EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.1 | expression in Escherichia coli strain BL21 | Pyrococcus horikoshii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.1 | purified recombinant enzyme, 20 mg/ml protien hanging drop vapour diffusion method, 3,8% PEG 4000, 0.1 M Na-acetate, pH 5.0-5.2, and 0.02 M MgCl2, asymmetric trimeric crystals, dimerization to hexamers, X-ray diffraction structure determination and analysis at 2.66 A resolution | Pyrococcus horikoshii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | Pyrococcus horikoshii | - |
2 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.1 | Pyrococcus horikoshii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.1 | recombinant enzyme from Escherichia coli strain BL21 | Pyrococcus horikoshii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.6.1.1 | diphosphate + H2O = 2 phosphate | active site structure | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | - |
Pyrococcus horikoshii | 2 phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.1 | hexamer | dimeric trimer, crystal structure | Pyrococcus horikoshii |
3.6.1.1 | More | structural basis for the high thermostability | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.1 | hyperthermophilic inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
3.6.1.1 | inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
3.6.1.1 | Pho-PPase | - |
Pyrococcus horikoshii |
3.6.1.1 | PPase | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | additional information | - |
optimal growth at 88°C | Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | additional information | - |
structural basis for the high thermostability | Pyrococcus horikoshii |