BRENDA - Enzyme Database

Substrate specificity analysis and inhibitor design of homoisocitrate dehydrogenase

Yamamoto, T.; Miyazaki, K.; Eguchi, T.; Bioorg. Med. Chem. 15, 1346-1355 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.87
expression in Escherichia coli
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.87
(R,E)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,E)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
3-vinylmalate
-
Deinococcus radiodurans
1.1.1.87
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview
Deinococcus radiodurans
1.1.1.87
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview, no inhibition by 3-vinylmalate
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.87
0.018
-
homoisocitrate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.083
-
3-vinylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.211
-
homoisocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.29
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.291
-
isocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.41
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.44
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.47
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.51
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.58
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.59
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.6
-
ethylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.78
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
1
-
ethylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
1.33
-
isopropylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
2.1
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
2.7
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
2.8
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
3.4
-
3-vinylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
9.4
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.87
Mg2+
-
Deinococcus radiodurans
1.1.1.87
Mg2+
-
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.87
Deinococcus radiodurans
-
-
-
1.1.1.87
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.87
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.87
additional information
-
substrate specificity, recombinant enzyme, overview
Deinococcus radiodurans
1.1.1.87
additional information
-
substrate specificity, recombinant enzyme, overview
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.87
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
3-vinylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
3-vinylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
ethylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
ethylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
homoisocitrate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
homoisocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
isocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
isopropylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
additional information
no activity with isocitrate, isopropylmalate, and tartrate
667934
Saccharomyces cerevisiae
?
-
-
-
-
1.1.1.87
additional information
no activity with tartrate
667934
Deinococcus radiodurans
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.87
28
-
assay at
Deinococcus radiodurans
1.1.1.87
36
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.87
0.14
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.34
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.37
-
isopropylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.5
-
3-vinylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.61
-
ethylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.7
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.77
-
3-vinylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.78
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
2
8
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
2.1
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
3
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
4.8
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
17
-
homoisocitrate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
25
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
29
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
37
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
43
-
ethylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
43
-
isocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
46
-
homoisocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
53
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.87
7.8
-
assay at
Deinococcus radiodurans
1.1.1.87
7.8
-
assay at
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.87
NAD+
-
Deinococcus radiodurans
1.1.1.87
NAD+
-
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.87
0.072
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.088
-
3-vinylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.26
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.51
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.79
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
1.4
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
3.1
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
5.2
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
15.3
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.87
expression in Escherichia coli
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.87
NAD+
-
Deinococcus radiodurans
1.1.1.87
NAD+
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.87
(R,E)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,E)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
1.1.1.87
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
1.1.1.87
3-vinylmalate
-
Deinococcus radiodurans
1.1.1.87
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview
Deinococcus radiodurans
1.1.1.87
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview, no inhibition by 3-vinylmalate
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.87
0.072
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.088
-
3-vinylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.26
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.51
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.79
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
1.4
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
3.1
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
5.2
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
15.3
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.87
0.018
-
homoisocitrate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.083
-
3-vinylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.211
-
homoisocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.29
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.291
-
isocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.41
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.44
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.47
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.51
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.58
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.59
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.6
-
ethylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.78
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
1
-
ethylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
1.33
-
isopropylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
2.1
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
2.7
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
2.8
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
3.4
-
3-vinylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
9.4
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.87
Mg2+
-
Deinococcus radiodurans
1.1.1.87
Mg2+
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.87
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.87
additional information
-
substrate specificity, recombinant enzyme, overview
Deinococcus radiodurans
1.1.1.87
additional information
-
substrate specificity, recombinant enzyme, overview
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.87
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
3-vinylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
3-vinylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
ethylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
ethylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
homoisocitrate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
1.1.1.87
homoisocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
isocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
isopropylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
1.1.1.87
additional information
no activity with isocitrate, isopropylmalate, and tartrate
667934
Saccharomyces cerevisiae
?
-
-
-
-
1.1.1.87
additional information
no activity with tartrate
667934
Deinococcus radiodurans
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.87
28
-
assay at
Deinococcus radiodurans
1.1.1.87
36
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.87
0.14
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.34
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.37
-
isopropylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.5
-
3-vinylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.61
-
ethylmalate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.7
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
0.77
-
3-vinylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
0.78
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
2
8
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
2.1
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
3
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
4.8
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
17
-
homoisocitrate
pH 7.8, 36°C, recombinant enzyme
Saccharomyces cerevisiae
1.1.1.87
25
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
29
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
37
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
43
-
ethylmalate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
43
-
isocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
46
-
homoisocitrate
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
1.1.1.87
53
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28°C, recombinant enzyme
Deinococcus radiodurans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.87
7.8
-
assay at
Deinococcus radiodurans
1.1.1.87
7.8
-
assay at
Saccharomyces cerevisiae