Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Izidoro, L.F.; Ribeiro, M.C.; Souza, G.R.; SantAna, C.D.; Hamaguchi, A.; Homsi-Brandeburgo, M.I.; Goulart, L.R.; Beleboni, R.O.; Nomizo, A.; Sampaio, S.V.; Soares, A.M.; Rodrigues, V.M.
    Biochemical and functional characterization of an L-amino acid oxidase isolated from Bothrops pirajai snake venom (2006), Bioorg. Med. Chem., 14, 7034-7043.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 66000
-
 2 * 66000, SDS-PAGE Bothrops pirajai
1.4.3.2 130000
-
 gel filtration Bothrops pirajai

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 additional information Bothrops pirajai enzyme induces time-dependent platelet aggregation, mouse paw edema, shows cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bothrops pirajai
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 glycoprotein deglycosylation does not alter enzymatic activity Bothrops pirajai

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2
-
 Bothrops pirajai

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bothrops pirajai
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 300
-
 substrate L-histidine, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 410
-
 substrate L-valine, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 435
-
 substrate L-isoleucine, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 440
-
 substrate L-methionine, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 580
-
 substrate L-leucine, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 585
-
 substrate L-tryptophan, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 685
-
 substrate L-tyrosine, 25°C, pH 7.2 Bothrops pirajai
1.4.3.2 765
-
 substrate L-phenylalanine, 25°C, pH 7.2 Bothrops pirajai

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-histidine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-isoleucine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-tryptophan + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai indole-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-tyrosine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai p-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-valine + H2O + O2 substrate affinity in decreasing order: L-phenylalanine, L-tyrosine, L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-valine, L-histidine Bothrops pirajai 2-oxoisovalerate + NH3 + H2O2
-
 ?
1.4.3.2 additional information enzyme induces time-dependent platelet aggregation, mouse paw edema, shows cytotoxic activity against Escherichia coli, Pseudomonas aeruginosa, Leishmania sp., and tumor cells Bothrops pirajai ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 dimer 2 * 66000, SDS-PAGE Bothrops pirajai

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 Bpir-LAOO-1
-
 Bothrops pirajai

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 37
-
-
 Bothrops pirajai

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 6 7.4
-
 Bothrops pirajai

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Bothrops pirajai isoelectric focusing
-
 4.9