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Literature summary extracted from

  • Iwaki-Egawa, S.; Yamamoto, T.; Watanabe, Y.
    Human plasma adenosine deaminase 2 is secreted by activated monocytes (2006), Biol. Chem., 387, 319-321.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.4 2'-deoxyformycin 50% inhibition of isozyme ADA2 at 0.09 mM Homo sapiens
3.5.4.4 erythro-9-(2-hydroxy-3-nonyl)adenine i.e. EHNA, specific for isozyme ADA1, no inhibition of isozyme ADA2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.4 additional information
-
 additional information isozymes ADA1 and ADA2 are kinetically distinct Homo sapiens
3.5.4.4 2.15
-
 adenosine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.4 extracellular isoyzme ADA2 is secreted by activated monocytes to blood plasma Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.4 67000
-
 2 * 67000, SDS-PAGE Homo sapiens
3.5.4.4 110000
-
 isozyme ADA2, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.4 2'-deoxyadenosine + H2O Homo sapiens the enzyme plays an important role in the immune system 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 adenosine + H2O Homo sapiens the enzyme plays an important role in the immune system inosine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.4 Homo sapiens
-
 isozymes ADA1 and ADA2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.4 isozyme ADA2 400fold from plasma by ammonium sulfate fractionation, anion exchange chromatography, adsorption chromatography, and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.4 B-cell isozyme ADA1 Homo sapiens
-
3.5.4.4 blood plasma isozyme ADA2 Homo sapiens
-
3.5.4.4 monocyte of peripheral blood Homo sapiens
-
3.5.4.4 additional information distribution of isozymes Homo sapiens
-
3.5.4.4 T-lymphocyte isoyzme ADA1 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.4 1.2
-
 purified isozyme ADA2 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.4 2'-deoxyadenosine + H2O
-
 Homo sapiens 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 2'-deoxyadenosine + H2O the enzyme plays an important role in the immune system Homo sapiens 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Homo sapiens inosine + NH3
-
 ?
3.5.4.4 adenosine + H2O the enzyme plays an important role in the immune system Homo sapiens inosine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.4 dimer 2 * 67000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.4 ADA2
-
 Homo sapiens
3.5.4.4 adenosine deaminase 2
-
 Homo sapiens