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Literature summary extracted from

  • Kim, S.; Rahman, A.; Mason, J.T.; Hirasawa, M.; Conover, R.C.; Johnson, M.K.; Miginiac-Maslow, M.; Keryer, E.; Knaff, D.B.; Leustek, T.
    The interaction of 5-adenylylsulfate reductase from Pseudomonas aeruginosa with its substrates (2005), Biochim. Biophys. Acta, 1710, 103-112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.99.2 expressed in Escherichia coli Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.10 C139S no activity Pseudomonas aeruginosa
1.8.4.10 C140S mutant enzyme shows 3% of wild-type activity Pseudomonas aeruginosa
1.8.4.10 C228S no activity Pseudomonas aeruginosa
1.8.4.10 C231S no activity Pseudomonas aeruginosa
1.8.4.10 C256S no activity Pseudomonas aeruginosa
1.8.99.2 C139S no detectable activity Pseudomonas aeruginosa
1.8.99.2 C140S 3% activity of the wild type enzyme Pseudomonas aeruginosa
1.8.99.2 C228S no detectable activity Pseudomonas aeruginosa
1.8.99.2 C231S no detectable activity Pseudomonas aeruginosa
1.8.99.2 C256S no detectable activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.99.2 2.7
-
 thioredoxin thioredoxin originating from Clamydomonas reinhardtii Pseudomonas aeruginosa
1.8.99.2 8.6
-
 thioredoxin thioredoxin originating from Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 30470
-
 MALDI-TOF mass spectrometry, the enzyme used is a recombinant protein with 48 additional residues containing the His6 tag Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.10 Pseudomonas aeruginosa
-
-
-
1.8.99.2 Pseudomonas aeruginosa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.99.2 0.302
-
 mutant enzyme C140S, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS Pseudomonas aeruginosa
1.8.99.2 10.3
-
 wild type enzyme, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 adenylylsulfate + thioredoxin
-
 Pseudomonas aeruginosa AMP + sulfite + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.99.2 monomer SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.8.4.10 PaAPR
-
 Pseudomonas aeruginosa
1.8.99.2 5'-adenylylsulfate reductase
-
 Pseudomonas aeruginosa
1.8.99.2 APR
-
 Pseudomonas aeruginosa