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Literature summary extracted from

  • Kulakova, L.; Galkin, A.; Nakayama, T.; Nishino, T.; Esaki, N.
    Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly->Pro substitution near the active site on its catalytic activity and stability (2004), Biochim. Biophys. Acta, 1696, 59-65.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.1 G244P significant increase in thermostability at 40°C, shift of acyl-chain preference towards higher preference for short-chain fqtty acid esters Psychrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.04
-
 4-nitrophenyl octanoate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 0.07
-
 4-nitrophenyl hexanoate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 0.19
-
 4-nitrophenyl valerate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 0.25
-
 4-nitrophenyl hexanoate mutant G244P, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 0.28
-
 4-nitrophenyl butanoate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 0.33
-
 4-nitrophenyl valerate mutant G244P, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 0.42
-
 4-nitrophenyl butanoate mutant G244P, pH 8.0, 25°C Psychrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Psychrobacter sp.
-
-
-
3.1.1.1 Psychrobacter sp. Ant300
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Psychrobacter sp. 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Psychrobacter sp. Ant300 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Psychrobacter sp. 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Psychrobacter sp. Ant300 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Psychrobacter sp. 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Psychrobacter sp. Ant300 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl valerate + H2O
-
 Psychrobacter sp. 4-nitrophenol + valerate
-
 ?
3.1.1.1 4-nitrophenyl valerate + H2O
-
 Psychrobacter sp. Ant300 4-nitrophenol + valerate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 40
-
 pH 7.9, wild-type, half-life 16 min, mutant G244P, half-life 11.6 h Psychrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 19
-
 4-nitrophenyl hexanoate mutant G244P, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 24
-
 4-nitrophenyl octanoate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 58
-
 4-nitrophenyl hexanoate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 74
-
 4-nitrophenyl valerate mutant G244P, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 110
-
 4-nitrophenyl butanoate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 110
-
 4-nitrophenyl valerate wild-type, pH 8.0, 25°C Psychrobacter sp.
3.1.1.1 110
-
 4-nitrophenyl butanoate mutant G244P, pH 8.0, 25°C Psychrobacter sp.