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Literature summary extracted from
- pH Stability of penicillin acylase from Escherichia coli (2004), Biochemistry (Moscow), 69, 1386-1390.
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | 6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O | - |
Escherichia coli | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
? |  |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | additional information | - |
study on inactivation kinetics over a wide pH-range at 25°C and 50°C. At temperatures above 35°C, enzyme undergoes a conformational change accompanied by destruction of salt bridges that maintain the active conformation | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 16 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 10.0, 25°C, ionic strength 0.12 M | Escherichia coli | |
| 3.5.1.11 | 26 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 9.1, 25°C, ionic strength 0.12 M | Escherichia coli | |
| 3.5.1.11 | 27 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 8.0, 25°C, ionic strength 0.12 M | Escherichia coli | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | additional information | - |
study on inactivation kinetics over a wide pH-range at 25°C and 50°C. Enzyme is very stable in neutral solutions and quickly loses its catalytic activity in acidic and alkaline solutions. Inactivation proceeds according to first order reaction kinetics | Escherichia coli |
| 3.5.1.11 | 8 | - |
half-life at 25°C is 6 days | Escherichia coli |
| 3.5.1.11 | 9.1 | - |
half-life at 25°C is 9.1 h | Escherichia coli |
| 3.5.1.11 | 10 | - |
half-life at 25°C is 0.9 h | Escherichia coli |
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