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Literature summary extracted from

  • Eprintsev, A.T.; Falaleeva, M.I.; Parfyonova, N.V.
    Malate dehydrogenase from the thermophilic bacterium Vulcanithermus medioatlanticus (2005), Biochemistry, 70, 1027-1030.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 citrate competitive with respect to oxaloacetate Vulcanithermus mediatlanticus
1.1.1.37 Mn2+ 2-40 mM Vulcanithermus mediatlanticus
1.1.1.37 oxaloacetate
-
 Vulcanithermus mediatlanticus
1.1.1.37 Zn2+ 0.05-0.5 mM Vulcanithermus mediatlanticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0014
-
 NADH
-
 Vulcanithermus mediatlanticus
1.1.1.37 0.048
-
 oxaloacetate
-
 Vulcanithermus mediatlanticus
1.1.1.37 0.4
-
 NAD+
-
 Vulcanithermus mediatlanticus
1.1.1.37 1.7
-
 malate
-
 Vulcanithermus mediatlanticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.37 Ba2+ 4-40 mM, activates Vulcanithermus mediatlanticus
1.1.1.37 Ca2+ 4-40 mM, activates Vulcanithermus mediatlanticus
1.1.1.37 Mg2+ 4-40 mM, activates Vulcanithermus mediatlanticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 55000
-
 x * 55000, SDS-PAGE Vulcanithermus mediatlanticus
1.1.1.37 128000
-
 gel filtration Vulcanithermus mediatlanticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Vulcanithermus mediatlanticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Vulcanithermus mediatlanticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 6.94
-
-
 Vulcanithermus mediatlanticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Vulcanithermus mediatlanticus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Vulcanithermus mediatlanticus oxaloacetate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 ? x * 55000, SDS-PAGE Vulcanithermus mediatlanticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 82
-
-
 Vulcanithermus mediatlanticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.37 60 90 60°C: about 45% of maximal activity, 90°C: about 60% of maximal activity Vulcanithermus mediatlanticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 50
-
 15 min, stable Vulcanithermus mediatlanticus
1.1.1.37 60
-
 15 min, about 25% loss of activity Vulcanithermus mediatlanticus
1.1.1.37 70
-
 15 min, about 50% loss of activity Vulcanithermus mediatlanticus
1.1.1.37 80
-
 15 min, about 70% loss of activity Vulcanithermus mediatlanticus
1.1.1.37 85
-
 1 min, complete loss of activity Vulcanithermus mediatlanticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 6.7
-
 reduction of oxaloacetate Vulcanithermus mediatlanticus
1.1.1.37 8.5
-
 oxidation of malate Vulcanithermus mediatlanticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.37 0.0205
-
 citrate
-
 Vulcanithermus mediatlanticus
1.1.1.37 0.37
-
 Zn2+
-
 Vulcanithermus mediatlanticus
1.1.1.37 2.1
-
 oxaloacetate
-
 Vulcanithermus mediatlanticus
1.1.1.37 2.5
-
 Mn2+
-
 Vulcanithermus mediatlanticus