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Literature summary extracted from

  • Vasil'eva, I.A.; Favre, A.; Lavrik, O.I.; Moor, N.A.
    Effect of nucleotide replacement in tRNAPhe on positioning of the acceptor end in the complex with phenylalanine-tRNA synthetase (2004), Biochemistry, 69, 154-163.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Thermus thermophilus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe effect of nucleotide replacement in tRNAPhe on positioning of the acceptor end in the complex with phenylalanine-tRNA synthetase Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
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Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 PheRS
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Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 additional information
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additional information turnover-number of tRNAPheG34A is 10% of wild-type value, turnover-number of tRNAPheG34C/A35U is 31% of wild-type value, turnover-number of tRNAPheA36C is 4.2% of wild-type value, turnover-number of tRNAPheU20A is identical to wild-type value, turnover-number of tRNAPheG44C is 2.1% of wild-type value, turnover-number of tRNAPheG19U/C56G is 1.7% of wild-type value Thermus thermophilus