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Literature summary extracted from

  • Kim, S.K.; Rahman, A.; Conover, R.C.; Johnson, M.K.; Mason, J.T.; Gomes, V.; Hirasawa, M.; Moore, M.L.; Leustek, T.; Knaff, D.B.
    Properties of the cysteine residues and the iron-sulfur cluster of the assimilatory 5-adenylyl sulfate reductase from Enteromorpha intestinalis (2006), Biochemistry, 45, 5010-5018.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.99.2 Na2SO4 100 mM Ulva intestinalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.6 expressed in Escherichia coli Ulva intestinalis
1.8.99.2 expressed in Escherichia coli Ulva intestinalis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.99.2 C165S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C166S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C250S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C257S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C260S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C285S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C342S reduced activity compared to wild type enzyme Ulva intestinalis
1.8.99.2 C345S reduced activity compared to wild type enzyme Ulva intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 48000
-
 non-reducing and reducing SDS-PAGE Ulva intestinalis
1.8.99.2 96000
-
 non-reducing SDS-PAGE, 16% of the protein was present as a homodimer Ulva intestinalis

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.6 Ulva intestinalis
-
-
-
1.8.99.2 Ulva intestinalis O81350
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.6 Ni2+ affinity column chromatography Ulva intestinalis
1.8.99.2 Ni2+ affinity chromatography Ulva intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.6 180
-
-
 Ulva intestinalis
1.8.99.2 0.011
-
 mutant enzyme 342S Ulva intestinalis
1.8.99.2 0.15
-
 mutant enzyme 345S Ulva intestinalis
1.8.99.2 3.5
-
 mutant enzyme C250S Ulva intestinalis
1.8.99.2 7.248
-
 wild type enzyme Ulva intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.6 L-cystine + glutathione
-
 Ulva intestinalis L-cysteine + GSSG
-
 ?
1.8.99.2 5'-adenylylsulfate + dithiothreitol
-
 Ulva intestinalis adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
 ir

Subunits

EC Number Subunits Comment Organism
1.8.99.2 homodimer 2 * 48000, non-reducing SDS-PAGE, 16% of the protein is present as a homodimer Ulva intestinalis
1.8.99.2 monomer 1 * 48000, non-reducing and reducing SDS-PAGE Ulva intestinalis

Synonyms

EC Number Synonyms Comment Organism
1.8.99.2 5'-adenylyl sulfate reductase
-
 Ulva intestinalis
1.8.99.2 EiAPR
-
 Ulva intestinalis