EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.284 | expressed in Escherichia coli BL21 cells | Homo sapiens |
1.2.1.46 | for expression in Escherichia coli BL21 cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.46 | formaldehyde dehydrogenase-adenosine 5'-diphosphate ribose and E67L-NADH binary complexes are determined | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.284 | E67L | mutant in which Glu67 is substituted with Leu | Homo sapiens |
1.1.1.284 | R368L | mutant in which Arg368 is substituted with Leu | Homo sapiens |
1.2.1.46 | E67L | mutant shows, that Glu67 is critical for capturing the substrates for catalysis | Homo sapiens |
1.2.1.46 | R368L | mutant shows, that the predominant role of Arg-368 is in the binding of the coenzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | dodecanoic acid | - |
Homo sapiens | |
1.2.1.46 | dodecanoic acid | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.0002 | - |
NADH | wild type enzyme, pH 7.5 | Homo sapiens | |
1.1.1.284 | 0.0017 | - |
S-hydroxymethylglutathione | wild type enzyme, pH 7.5, 30°C | Homo sapiens | |
1.1.1.284 | 0.0033 | - |
NAD+ | wild type enzyme | Homo sapiens | |
1.1.1.284 | 0.0048 | - |
S-nitrosoglutathione | wild type enzyme, pH 7.5, 25°C | Homo sapiens | |
1.1.1.284 | 0.0093 | - |
NAD+ | wild type enzyme, pH 10 | Homo sapiens | |
1.1.1.284 | 0.011 | - |
NADH | mutant R368L, pH 7.5 | Homo sapiens | |
1.1.1.284 | 0.0144 | - |
NAD+ | mutant R368L, pH 7.5 | Homo sapiens | |
1.1.1.284 | 0.038 | - |
S-hydroxymethylglutathione | mutant R368L, pH 7.5, 30°C | Homo sapiens | |
1.1.1.284 | 0.041 | - |
12-Hydroxydodecanoic acid | wild type enzyme, pH 10 | Homo sapiens | |
1.1.1.284 | 0.054 | - |
NAD+ | mutant E67L, pH 10 | Homo sapiens | |
1.1.1.284 | 0.116 | - |
NAD+ | mutant E67L | Homo sapiens | |
1.1.1.284 | 0.16 | - |
NAD+ | mutant R368L, pH 10 | Homo sapiens | |
1.1.1.284 | 0.16 | - |
12-Hydroxydodecanoic acid | mutant R368L, pH 10 | Homo sapiens | |
1.1.1.284 | 0.16 | - |
S-nitrosoglutathione | mutant R368L, pH 7.5, 25°C | Homo sapiens | |
1.1.1.284 | 0.68 | - |
12-Hydroxydodecanoic acid | mutant E67L, pH 10 | Homo sapiens | |
1.1.1.284 | 2.65 | - |
S-hydroxymethylglutathione | mutant E67L, pH 7.5, 30°C | Homo sapiens | |
1.2.1.46 | 0.0017 | - |
S-hydroxymethylglutathione | oxidation of S-hydroxymethylglutathione, formaldehyde dehydrogenase | Homo sapiens | |
1.2.1.46 | 0.002 | - |
NADH | oxidation of NADH, formaldehyde dehydrogenase | Homo sapiens | |
1.2.1.46 | 0.0033 | - |
NAD+ | reduction of NAD+, formaldehyde dehydrogenase | Homo sapiens | |
1.2.1.46 | 0.0048 | - |
S-nitrosoglutathione | reduction of S-nitrosoglutathione, formaldehyde dehydrogenase | Homo sapiens | |
1.2.1.46 | 0.011 | - |
NADH | oxidation of NADH, R368L mutant | Homo sapiens | |
1.2.1.46 | 0.0144 | - |
NAD+ | reduction of NAD+, R368L mutant | Homo sapiens | |
1.2.1.46 | 0.038 | - |
S-hydroxymethylglutathione | oxidation of S-hydroxymethylglutathione, R368L mutant | Homo sapiens | |
1.2.1.46 | 0.116 | - |
NAD+ | reduction of NAD+, E67L mutant | Homo sapiens | |
1.2.1.46 | 0.16 | - |
S-nitrosoglutathione | reduction of S-nitrosoglutathione, R368L mutant | Homo sapiens | |
1.2.1.46 | 2.65 | - |
S-hydroxymethylglutathione | oxidation of S-hydroxymethylglutathione, E67L mutant | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.46 | Zn2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.46 | formaldehyde + NAD+ + H2O | Homo sapiens | - |
formate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.284 | Homo sapiens | - |
- |
- |
1.2.1.46 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.284 | DE52 column chromatography, Q-Sepharose column chromatography and Sephacryl S-100HR column chromatography | Homo sapiens |
1.2.1.46 | DE52, Q-sepharose and Sephacryl S-100HR chromatographic steps | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.284 | 12-hydroxydodecanoic acid + NAD+ | - |
Homo sapiens | ? + NADH | - |
? | |
1.1.1.284 | S-hydroxymethylglutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
? | |
1.1.1.284 | S-nitrosoglutathione + NADH | - |
Homo sapiens | GSH + NAD+ + NO | - |
? | |
1.2.1.46 | formaldehyde + NAD+ + H2O | - |
Homo sapiens | formate + NADH + H+ | - |
? | |
1.2.1.46 | S-hydroxymethylglutathione + NAD+ + H2O | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
? | |
1.2.1.46 | S-nitrosoglutathione + NADH + H2O | - |
Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.46 | homodimer | - |
Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.284 | FDH | - |
Homo sapiens |
1.1.1.284 | Glutathione-dependent formaldehyde dehydrogenase | - |
Homo sapiens |
1.2.1.46 | FDH | - |
Homo sapiens |
1.2.1.46 | formaldehyde dehydrogenase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.46 | 25 | - |
S-nitrosoglutathione | Homo sapiens |
1.2.1.46 | 30 | - |
S-hydroxymethylglutathione | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.92 | - |
NAD+ | mutant E67L, pH 7.5 | Homo sapiens | |
1.1.1.284 | 1.4 | - |
S-hydroxymethylglutathione | mutant E67L, pH 7.5, 30°C | Homo sapiens | |
1.1.1.284 | 2.5 | - |
NAD+ | wild type enzyme, pH 7.5 | Homo sapiens | |
1.1.1.284 | 2.5 | - |
S-hydroxymethylglutathione | wild type enzyme, pH 7.5, 30°C | Homo sapiens | |
1.1.1.284 | 15.3 | - |
S-nitrosoglutathione | mutant R368L, pH 7.5, 25°C | Homo sapiens | |
1.1.1.284 | 16.7 | - |
NAD+ | mutant R368L, pH 7.5 | Homo sapiens | |
1.1.1.284 | 20 | - |
S-hydroxymethylglutathione | mutant R368L, pH 7.5, 30°C | Homo sapiens | |
1.1.1.284 | 29.3 | - |
S-nitrosoglutathione | wild type enzyme, pH 7.5, 25°C | Homo sapiens | |
1.1.1.284 | 34.5 | - |
NADH | wild type enzyme, pH 7.5 | Homo sapiens | |
1.1.1.284 | 203.3 | - |
S-nitrosoglutathione | mutant R368L, pH 7.5, 25°C | Homo sapiens | |
1.1.1.284 | 230 | - |
NADH | mutant R368L, pH 7.5 | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.46 | 7.5 | - |
- |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | NAD+ | - |
Homo sapiens | |
1.2.1.46 | NADH | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.148 | - |
dodecanoic acid | wild type enzyme | Homo sapiens | |
1.1.1.284 | 0.161 | - |
dodecanoic acid | mutant R368L | Homo sapiens | |
1.1.1.284 | 0.617 | - |
dodecanoic acid | mutant E67L | Homo sapiens | |
1.2.1.46 | 0.148 | - |
dodecanoic acid | formaldehyde dehydrogenase | Homo sapiens | |
1.2.1.46 | 0.161 | - |
dodecanoic acid | R368L mutant | Homo sapiens | |
1.2.1.46 | 0.617 | - |
dodecanoic acid | E67L mutant | Homo sapiens |