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Literature summary extracted from

  • Sanghani, P.C.; Davis, W.I.; Zhai, L.; Robinson, H.
    Structure-function relationships in human glutathione-dependent formaldehyde dehydrogenase. Role of Glu-67 and Arg-368 in the catalytic mechanism (2006), Biochemistry, 45, 4819-4830.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.284 expressed in Escherichia coli BL21 cells Homo sapiens
1.2.1.46 for expression in Escherichia coli BL21 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.46 formaldehyde dehydrogenase-adenosine 5'-diphosphate ribose and E67L-NADH binary complexes are determined Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.284 E67L mutant in which Glu67 is substituted with Leu Homo sapiens
1.1.1.284 R368L mutant in which Arg368 is substituted with Leu Homo sapiens
1.2.1.46 E67L mutant shows, that Glu67 is critical for capturing the substrates for catalysis Homo sapiens
1.2.1.46 R368L mutant shows, that the predominant role of Arg-368 is in the binding of the coenzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 dodecanoic acid
-
Homo sapiens
1.2.1.46 dodecanoic acid
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.0002
-
NADH wild type enzyme, pH 7.5 Homo sapiens
1.1.1.284 0.0017
-
S-hydroxymethylglutathione wild type enzyme, pH 7.5, 30°C Homo sapiens
1.1.1.284 0.0033
-
NAD+ wild type enzyme Homo sapiens
1.1.1.284 0.0048
-
S-nitrosoglutathione wild type enzyme, pH 7.5, 25°C Homo sapiens
1.1.1.284 0.0093
-
NAD+ wild type enzyme, pH 10 Homo sapiens
1.1.1.284 0.011
-
NADH mutant R368L, pH 7.5 Homo sapiens
1.1.1.284 0.0144
-
NAD+ mutant R368L, pH 7.5 Homo sapiens
1.1.1.284 0.038
-
S-hydroxymethylglutathione mutant R368L, pH 7.5, 30°C Homo sapiens
1.1.1.284 0.041
-
12-Hydroxydodecanoic acid wild type enzyme, pH 10 Homo sapiens
1.1.1.284 0.054
-
NAD+ mutant E67L, pH 10 Homo sapiens
1.1.1.284 0.116
-
NAD+ mutant E67L Homo sapiens
1.1.1.284 0.16
-
NAD+ mutant R368L, pH 10 Homo sapiens
1.1.1.284 0.16
-
12-Hydroxydodecanoic acid mutant R368L, pH 10 Homo sapiens
1.1.1.284 0.16
-
S-nitrosoglutathione mutant R368L, pH 7.5, 25°C Homo sapiens
1.1.1.284 0.68
-
12-Hydroxydodecanoic acid mutant E67L, pH 10 Homo sapiens
1.1.1.284 2.65
-
S-hydroxymethylglutathione mutant E67L, pH 7.5, 30°C Homo sapiens
1.2.1.46 0.0017
-
S-hydroxymethylglutathione oxidation of S-hydroxymethylglutathione, formaldehyde dehydrogenase Homo sapiens
1.2.1.46 0.002
-
NADH oxidation of NADH, formaldehyde dehydrogenase Homo sapiens
1.2.1.46 0.0033
-
NAD+ reduction of NAD+, formaldehyde dehydrogenase Homo sapiens
1.2.1.46 0.0048
-
S-nitrosoglutathione reduction of S-nitrosoglutathione, formaldehyde dehydrogenase Homo sapiens
1.2.1.46 0.011
-
NADH oxidation of NADH, R368L mutant Homo sapiens
1.2.1.46 0.0144
-
NAD+ reduction of NAD+, R368L mutant Homo sapiens
1.2.1.46 0.038
-
S-hydroxymethylglutathione oxidation of S-hydroxymethylglutathione, R368L mutant Homo sapiens
1.2.1.46 0.116
-
NAD+ reduction of NAD+, E67L mutant Homo sapiens
1.2.1.46 0.16
-
S-nitrosoglutathione reduction of S-nitrosoglutathione, R368L mutant Homo sapiens
1.2.1.46 2.65
-
S-hydroxymethylglutathione oxidation of S-hydroxymethylglutathione, E67L mutant Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.46 Zn2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O Homo sapiens
-
formate + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Homo sapiens
-
-
-
1.2.1.46 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284 DE52 column chromatography, Q-Sepharose column chromatography and Sephacryl S-100HR column chromatography Homo sapiens
1.2.1.46 DE52, Q-sepharose and Sephacryl S-100HR chromatographic steps Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 12-hydroxydodecanoic acid + NAD+
-
Homo sapiens ? + NADH
-
?
1.1.1.284 S-hydroxymethylglutathione + NAD+
-
Homo sapiens S-formylglutathione + NADH + H+
-
?
1.1.1.284 S-nitrosoglutathione + NADH
-
Homo sapiens GSH + NAD+ + NO
-
?
1.2.1.46 formaldehyde + NAD+ + H2O
-
Homo sapiens formate + NADH + H+
-
?
1.2.1.46 S-hydroxymethylglutathione + NAD+ + H2O
-
Homo sapiens S-formylglutathione + NADH + H+
-
?
1.2.1.46 S-nitrosoglutathione + NADH + H2O
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.46 homodimer
-
Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 FDH
-
Homo sapiens
1.1.1.284 Glutathione-dependent formaldehyde dehydrogenase
-
Homo sapiens
1.2.1.46 FDH
-
Homo sapiens
1.2.1.46 formaldehyde dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.46 25
-
S-nitrosoglutathione Homo sapiens
1.2.1.46 30
-
S-hydroxymethylglutathione Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 0.92
-
NAD+ mutant E67L, pH 7.5 Homo sapiens
1.1.1.284 1.4
-
S-hydroxymethylglutathione mutant E67L, pH 7.5, 30°C Homo sapiens
1.1.1.284 2.5
-
NAD+ wild type enzyme, pH 7.5 Homo sapiens
1.1.1.284 2.5
-
S-hydroxymethylglutathione wild type enzyme, pH 7.5, 30°C Homo sapiens
1.1.1.284 15.3
-
S-nitrosoglutathione mutant R368L, pH 7.5, 25°C Homo sapiens
1.1.1.284 16.7
-
NAD+ mutant R368L, pH 7.5 Homo sapiens
1.1.1.284 20
-
S-hydroxymethylglutathione mutant R368L, pH 7.5, 30°C Homo sapiens
1.1.1.284 29.3
-
S-nitrosoglutathione wild type enzyme, pH 7.5, 25°C Homo sapiens
1.1.1.284 34.5
-
NADH wild type enzyme, pH 7.5 Homo sapiens
1.1.1.284 203.3
-
S-nitrosoglutathione mutant R368L, pH 7.5, 25°C Homo sapiens
1.1.1.284 230
-
NADH mutant R368L, pH 7.5 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.46 7.5
-
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
Homo sapiens
1.2.1.46 NADH
-
Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.284 0.148
-
dodecanoic acid wild type enzyme Homo sapiens
1.1.1.284 0.161
-
dodecanoic acid mutant R368L Homo sapiens
1.1.1.284 0.617
-
dodecanoic acid mutant E67L Homo sapiens
1.2.1.46 0.148
-
dodecanoic acid formaldehyde dehydrogenase Homo sapiens
1.2.1.46 0.161
-
dodecanoic acid R368L mutant Homo sapiens
1.2.1.46 0.617
-
dodecanoic acid E67L mutant Homo sapiens