EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.9 | expressed in Escherichia coli strain BL21(DE3)-pLysS | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.9 | 0.00057 | - |
5'-adenylylsulfate | assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C | Mycobacterium tuberculosis | |
1.8.4.9 | 0.031 | - |
3'-phosphoadenosine-5'-phosphosulfate | assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.9 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.9 | Sepharose column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.9 | 3'-phosphoadenosine-5'-phosphosulfate + glutathione | - |
Mycobacterium tuberculosis | adenosine 3',5'-bisphosphate + sulfite + oxidized glutathione | - |
? | |
1.8.4.9 | 5'-adenylylsulfate + glutathione + glutathione | - |
Mycobacterium tuberculosis | adenosine monophosphate + sulfite + oxidized glutathione | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.9 | APS reductase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.9 | 0.012 | - |
3'-phosphoadenosine-5'-phosphosulfate | assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C | Mycobacterium tuberculosis | |
1.8.4.9 | 0.153 | - |
5'-adenylylsulfate | assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C | Mycobacterium tuberculosis |