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Literature summary extracted from

  • Adler, M.; Bryant, J.; Buckman, B.; Islam, I.; Larsen, B.; Finster, S.; Kent, L.; May, K.; Mohan, R.; Yuan, S.; Whitlow, M.
    Crystal structures of potent thiol-based inhibitors bound to carboxypeptidase B (2005), Biochemistry, 44, 9339-9347.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.17.2 enzyme in complex with inhibitors DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid, 2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid, (2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid, or 5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid, hanging drop vapour diffusion method, 0.21 mg/ml protein in 10 mM Tris, pH 7.0, 1 mM inhibitor, and 50 mM NaCl, mixed with an equal volume of 0.002 ml of reservoir solution containing 0.15-0.30 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, and 12-22% PEG 8000, X-ray diffraction structure determination and analysis at 1.4-2.0 A resolution Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.2 (2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid binding structure analysis Sus scrofa
3.4.17.2 2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid binding structure analysis Sus scrofa
3.4.17.2 5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid binding structure analysis Sus scrofa
3.4.17.2 DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid binding structure analysis Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.2 Sus scrofa P09955
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.2 further purification of the commercial pancreatic preparation by hydrophobic interaction chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.2 commercial preparation
-
 Sus scrofa
-
3.4.17.2 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.2 hippuryl-L-arginine + H2O
-
 Sus scrofa hippuric acid + L-Arg
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.2 pp-CpB
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.2 22
-
 assay at room temperature Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.2 7.4
-
 assay at Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.17.2 0.000002
-
 (2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid pH 7.4, 22°C Sus scrofa
3.4.17.2 0.000002
-
 5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid pH 7.4, 22°C Sus scrofa
3.4.17.2 0.000007
-
 2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid pH 7.4, 22°C Sus scrofa
3.4.17.2 0.000063
-
 DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid pH 7.4, 22°C Sus scrofa