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Literature summary extracted from

  • Wen, T.N.; Chen, J.L.; Lee, S.H.; Yang, N.S.; Shyur, L.F.
    A truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase with improved enzymatic activity and thermotolerance (2005), Biochemistry, 44, 9197-9205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 C-terminally truncated (10 kDa), overexpression in Escherichia coli BL21 (DE3) and Pichia pastoris X-33, mutants with increased catalytic efficiency and thermotolerance Fibrobacter succinogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 additional information resistent to trypsin protease digestion Fibrobacter succinogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 2.5
-
 lichenan wild type Fibrobacter succinogenes
3.2.1.73 2.83
-
 lichenan expressed in Pichia pastoris Fibrobacter succinogenes
3.2.1.73 2.89
-
 lichenan expressed in Escherichia coli Fibrobacter succinogenes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Fibrobacter succinogenes
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.73 glycoprotein expressed in Pichia pastoris Fibrobacter succinogenes

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 Q-Sepharose FF column twice, Ni-NTA affinity column for the Escherichia coli protein, Q-Sepharose FF column for the Pichia pastoris protein Fibrobacter succinogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 2065
-
 wild type, sodium citrate buffer, pH 6.0, 50°C, 10 min Fibrobacter succinogenes
3.2.1.73 7980
-
 expressed in Escherichia coli, sodium citrate buffer, pH 6.0, 50°C, 10 min Fibrobacter succinogenes
3.2.1.73 10800
-
 expressed in Pichia pastoris X-33, sodium citrate buffer, pH 6.0, 50°C, 10 min Fibrobacter succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 lichenan + H2O
-
 Fibrobacter succinogenes ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 Fsbeta-glucanase
-
 Fibrobacter succinogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 50
-
 pH 6.0 Fibrobacter succinogenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 30 55
-
 Fibrobacter succinogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 90
-
 80% activity after 10 min Fibrobacter succinogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6 7 recombinant proteins Fibrobacter succinogenes
3.2.1.73 6 8 wild type Fibrobacter succinogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 6 10
-
 Fibrobacter succinogenes