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Literature summary extracted from

  • Nguyen, K.T.; Pei, D.
    Purification and characterization of enzymes involved in the degradation of chemotactic N-formyl peptides (2005), Biochemistry, 44, 8514-8522.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 1,10-phenanthroline 6% inhibition at 10 mM Rattus norvegicus
3.5.1.14 DTT 85% inhibition at 20 mM Rattus norvegicus
3.5.1.14 EDTA 10% inhibition at 50 mM Rattus norvegicus
3.5.1.14 iodoacetamide 90% inhibition at 10 mM Rattus norvegicus
3.5.1.14 Nepsilon-formyl-Nepsilon-hydroxy-L-lysine IC50: 1.0 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.3
-
 N-acetylmethionine pH 8.0, 22°C Rattus norvegicus
3.5.1.14 3.1
-
 N-formylmethionine pH 8.0, 22°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 CoCl2 activates 2-3fold at 0.1 mM Rattus norvegicus
3.5.1.14 NiCl2 activates 2-3fold at 0.1 mM Rattus norvegicus
3.5.1.14 Zn2+ 0.98 Zn2+ per enzyme molecule, tightly bound, involved in cataylsis Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 45000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 additional information Rattus norvegicus the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Rattus norvegicus
-
 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 native enzyme from intestinal epithelial mucosa, 1833fold to homogeneity, by acid precipitation, two steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration and concanavalin A afinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 mucosa intestinal Rattus norvegicus
-
3.5.1.14 small intestine mucosa Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.14 additional information
-
-
 Rattus norvegicus
3.5.1.14 2.2
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 additional information the enzyme is involved in the degradation of chemotactic N-formyl peptides and plays a protective role in degrading bacterial and mitochondrial N-formylated peptide together with the alpha-N-acylpeptide hydrolase, EC 3.4.19.1 Rattus norvegicus ?
-
 ?
3.5.1.14 additional information no activity with N-formylmethionylalanine, N-formylalanylalanine, N-formylmethionylalanylserine, N-acetylmethionylalanylserine, and N-formylmethionylleucylphenylalanine Rattus norvegicus ?
-
 ?
3.5.1.14 N-acetylmethionine + H2O preferred substrate Rattus norvegicus acetate + L-methionine
-
 ?
3.5.1.14 N-formylmethionine + H2O substrate synthesis and purification, overview Rattus norvegicus formate + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 monomer 1 * 45000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 fMDF
-
 Rattus norvegicus
3.5.1.14 N-acylase IA
-
 Rattus norvegicus
3.5.1.14 N-formylmethionine deformylase
-
 Rattus norvegicus
3.5.1.14 Nalpha-acyl-L-amino acid amidohydrolase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 22
-
 assay at room temperature Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.14 2.3
-
 N-acetylmethionine pH 8.0, 22°C Rattus norvegicus
3.5.1.14 7.9
-
 N-formylmethionine pH 8.0, 22°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 8
-
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.14 1
-
 IC50: 1.0 mM Rattus norvegicus Nepsilon-formyl-Nepsilon-hydroxy-L-lysine