EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.19.5 | - |
Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.19.5 | hanging drop vapor diffusion method. X-ray crystal structure of the D285N mutant complexed with beta-Asp-His | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.19.5 | D285A | kcat/Km for beta-Asp-Leu is 85000fold lower than wild-type value | Escherichia coli |
3.4.19.5 | D285N | kcat/Km for beta-Asp-Leu is 5667fold lower than wild-type value | Escherichia coli |
3.4.19.5 | E77D | kcat/Km for beta-Asp-Leu is 137837fold lower than wild-type value | Escherichia coli |
3.4.19.5 | E77Q | kcat/Km for beta-Asp-Leu is 14571fold lower than wild-type value | Escherichia coli |
3.4.19.5 | R169K | kcat/Km for beta-Asp-Leu is 378fold lower than wild-type value | Escherichia coli |
3.4.19.5 | R169M | kcat/Km for beta-Asp-Leu is 1672131fold lower than wild-type value | Escherichia coli |
3.4.19.5 | R233K | kcat/Km for beta-Asp-Leu is 192fold lower than wild-type value | Escherichia coli |
3.4.19.5 | R233M | kcat/Km for beta-Asp-Leu is 170fold lower than wild-type value | Escherichia coli |
3.4.19.5 | S289A | kcat/Km for beta-Asp-Leu is 30000fold lower than wild-type value | Escherichia coli |
3.4.19.5 | Y137A | kcat/Km for beta-Asp-Leu is 927fold lower than wild-type value | Escherichia coli |
3.4.19.5 | Y137F | kcat/Km for beta-Asp-Leu is 850fold lower than wild-type value | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.5 | 0.09 | - |
beta-Asp-Leu | Ni/Ni reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.23 | - |
beta-Asp-Phe | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.36 | - |
beta-Asp-Leu | Cd/Cd reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.5 | - |
beta-Asp-Leu | mutant enzyme D285A, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.62 | - |
beta-Asp-Leu | Co/Co reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.8 | - |
beta-Asp-Leu | mutant enzyme E77Q, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.91 | - |
beta-Asp-Lys | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.98 | - |
beta-Asp-Leu | mutant enzyme D285N, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 1.02 | - |
beta-Asp-Leu | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 1.02 | - |
beta-Asp-Leu | Zn/Zn reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 1.4 | - |
beta-Asp-Leu | mutant enzyme Y137F, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 1.7 | - |
beta-Asp-Leu | mutant enzyme Y137A, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 2.7 | - |
beta-Asp-Leu | mutant enzyme S289A, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 3.7 | - |
beta-Asp-His | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 3.7 | - |
beta-Asp-Ala | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 5 | - |
alpha-Asp-Leu | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 6.9 | - |
beta-Asp-Leu | mutant enzyme E77D, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 18 | - |
beta-Asp-Gly | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 20 | - |
beta-Asp-Leu | mutant enzyme R233K, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 34 | - |
beta-Asp-Leu | mutant enzyme R169K, pH 8.1, 30°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.19.5 | additional information | the active site consists of a binuclear metal center positioned at the C-terminal end of a (beta/alpha)8-barrel domain | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.19.5 | Escherichia coli | P39377 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.19.5 | alpha-Asp-Leu + H2O | - |
Escherichia coli | L-Asp + L-Leu | - |
? | |
3.4.19.5 | beta-Asp-Ala + H2O | - |
Escherichia coli | Asp + Ala | - |
? | |
3.4.19.5 | beta-Asp-Gly + H2O | - |
Escherichia coli | Asp + Gly | - |
? | |
3.4.19.5 | beta-Asp-His + H2O | - |
Escherichia coli | Asp + His | - |
? | |
3.4.19.5 | beta-Asp-Leu + H2O | - |
Escherichia coli | Asp + Leu | - |
? | |
3.4.19.5 | beta-Asp-Lys + H2O | - |
Escherichia coli | Asp + Lys | - |
? | |
3.4.19.5 | beta-Asp-Phe + H2O | - |
Escherichia coli | Asp + Phe | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.19.5 | IAD | - |
Escherichia coli |
3.4.19.5 | isoaspartyl dipeptidase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.5 | 0.00062 | - |
beta-Asp-Leu | mutant enzyme D285A, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.0051 | - |
beta-Asp-Leu | mutant enzyme E77D, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.0056 | - |
beta-Asp-Leu | mutant enzyme E77Q, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.017 | - |
beta-Asp-Leu | mutant enzyme D285N, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.18 | - |
beta-Asp-Leu | mutant enzyme Y137F, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.19 | - |
beta-Asp-Leu | mutant enzyme Y137A, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 0.93 | - |
beta-Asp-Gly | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 9 | - |
beta-Asp-Leu | mutant enzyme R169K, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 9 | - |
beta-Asp-Leu | mutant enzyme S289A, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 9.2 | - |
beta-Asp-Leu | Ni/Ni reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 11.9 | - |
beta-Asp-Leu | Cd/Cd reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 13 | - |
beta-Asp-Leu | mutant enzyme R233K, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 15.7 | - |
alpha-Asp-Leu | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 16.9 | - |
beta-Asp-Phe | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 20.8 | - |
beta-Asp-His | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 34 | - |
beta-Asp-Leu | Co/Co reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 58 | - |
beta-Asp-Lys | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 104 | - |
beta-Asp-Leu | wild-type enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 104 | - |
beta-Asp-Leu | Zn/Zn reconstituted enzyme, pH 8.1, 30°C | Escherichia coli | |
3.4.19.5 | 213 | - |
beta-Asp-Ala | wild-type enzyme, pH 8.1, 30°C | Escherichia coli |