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Literature summary extracted from

  • Rossocha, M.; Schultz-Heienbrok, R.; von Moeller, H.; Coleman, J.P.; Saenger, W.
    Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product (2005), Biochemistry, 44, 5739-5748.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.24 enzyme in complex with substrate taurodeoxycholate and as apoenzyme, complexed enzyme by hanging drop vapour diffusion method, 18°C, mixing of 0.003 ml protein solution and 0.002 ml reservoir solution, the latter containing 2.6 M ammonium sulfate, 0.1 M sodium citrate, pH 6.0, and 1 mM taurodeoxycholate, 3 days, apoenzyme crystals by sitting drop vapour diffusion method, 18°C, mixing of 0.0015 ml protein solution with 0.001 ml reservoir solution containing 25% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M Bis-Tris, pH 5.5, 3 days, orthorhombic crystals in both cases, X-ray diffraction structure determination and analysis at 1.7 and 2.1 A resolution, respectively Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.24 taurodeoxycholate + H2O Clostridium perfringens
-
 taurine + deoxycholate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.24 Clostridium perfringens P54965
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.24 glycocholate + H2O = cholate + glycine substrate binding pocket and active site structure, taurine has a reversed orientation pointing with its sulfo group towards Cys2 and leaving the active site with its amino group ahead, overview Clostridium perfringens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.24 taurodeoxycholate + H2O
-
 Clostridium perfringens taurine + deoxycholate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.24 More computational structure comparisons Clostridium perfringens
3.5.1.24 tetramer the enzyme contains an N-terminal thiol hydrolase activity site, quarternary structure, crystal structure analysis, overview Clostridium perfringens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.24 bile salt hydrolase
-
 Clostridium perfringens
3.5.1.24 CBAH
-
 Clostridium perfringens
3.5.1.24 Conjugated bile acid hydrolase
-
 Clostridium perfringens