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Literature summary extracted from

  • Cheung, Y.Y.; Lam, S.Y.; Chu, W.K.; Allen, M.D.; Bycroft, M.; Wong, K.B.
    Crystal structure of a hyperthermophilic archaeal acylphosphatase from Pyrococcus horikoshii - structural insights into enzymatic catalysis, thermostability, and dimerization (2005), Biochemistry, 44, 4601-4611.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.7 expressed in Escherichia coli Pyrococcus horikoshii
3.6.1.7 expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.7 sitting-drop-vapor-diffusion method, 1.5 A crystal structure. The enzyme forms a dimer in the crystal structure via antiparallel association of strand 4 Pyrococcus horikoshii
3.6.1.7 sitting-drop-vapor-diffusion with sodium formate as precipitant at pH 6.0 Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.7 0.12
-
 benzoyl phosphate at 25°C and pH 5.3 Pyrococcus horikoshii
3.6.1.7 0.12
-
 benzoyl phosphate 25-45°C, pH 5.3 Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.7 10128
-
 1 * 10128, enzyme in solution Pyrococcus horikoshii
3.6.1.7 10128
-
 2 * 10128, in the crystal structure via antiparallel association of strand 4 with formation of nine interchain hydrogen bonds Pyrococcus horikoshii
3.6.1.7 10129
-
 1 * 10129, calculated from sequence, although the enzyme exists as a monomer in solution, it can dimerize via antiparallel association of strand 4, the protein forms a dimer in the crystal structure via antiparallel association of strand 4 Pyrococcus horikoshii
3.6.1.7 10130
-
 mass spectrometry Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Pyrococcus horikoshii P84142
-
-
3.6.1.7 Pyrococcus horikoshii DSM 12428 P84142
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii DSM 12428 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Pyrococcus horikoshii acetate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Pyrococcus horikoshii DSM 12428 acetate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Pyrococcus horikoshii benzoate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Pyrococcus horikoshii DSM 12428 benzoate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii carbamate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii DSM 12428 carbamate + phosphate
-
 ?
3.6.1.7 nucleoside diphosphate + H2O
-
 Pyrococcus horikoshii nucleoside phosphate + phosphate
-
 ?
3.6.1.7 nucleoside diphosphate + H2O
-
 Pyrococcus horikoshii DSM 12428 nucleoside phosphate + phosphate
-
 ?
3.6.1.7 nucleoside triphosphate + H2O
-
 Pyrococcus horikoshii nucleoside diphosphate + phosphate
-
 ?
3.6.1.7 succinyl phosphate + H2O
-
 Pyrococcus horikoshii succinate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.7 dimer 2 * 10128, in the crystal structure via antiparallel association of strand 4 with formation of nine interchain hydrogen bonds Pyrococcus horikoshii
3.6.1.7 monomer 1 * 10128, enzyme in solution Pyrococcus horikoshii
3.6.1.7 monomer 1 * 10129, calculated from sequence, although the enzyme exists as a monomer in solution, it can dimerize via antiparallel association of strand 4, the protein forms a dimer in the crystal structure via antiparallel association of strand 4 Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.6.1.7 PhAcP
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.7 98
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.7 25 45 turnover number increases 4fold by an increase of temperature from 25 to 45°C Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.7 25 100 At 25°C, the free energy of unfolding, midpoint of transition, and m value were 54.4 kJ/mol, 4.99 M, and 10.9 kJ/mol/M, respectively. The melting temperature is about 111.5°C. These data indicate that PhAcP is an extremely thermostable protein. Pyrococcus horikoshii
3.6.1.7 100
-
 stable up to Pyrococcus horikoshii
3.6.1.7 112
-
 melting temperature: 111.5°C Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.7 additional information
-
 additional information experimentally, it is impossible to assay the enzyme activity of the enzyme directly at 98°C because the uncatalyzed hydrolysis of the acyl-phosphate substrate is too fast at high temperatures to allow an accurate measurement of kinetic parameters. Instead, the kinetic parameters of the enzyme are measured at 25-45 °C and the kcat value of the enzyme at 98 °C is estimated by linear extrapolation of the Arrhenius plot. The estimated kcat value is 10000/s Pyrococcus horikoshii
3.6.1.7 93.5
-
 benzoyl phosphate at 25°C and pH 5.3 Pyrococcus horikoshii
3.6.1.7 93.5
-
 benzoyl phosphate 25°C, pH 5.3 Pyrococcus horikoshii
3.6.1.7 191
-
 benzoyl phosphate at 30°C and pH 5.3 Pyrococcus horikoshii
3.6.1.7 191
-
 benzoyl phosphate 30°C, pH 5.3 Pyrococcus horikoshii
3.6.1.7 275
-
 benzoyl phosphate at 35°C and pH 5.3 Pyrococcus horikoshii
3.6.1.7 275
-
 benzoyl phosphate 35°C, pH 5.3 Pyrococcus horikoshii
3.6.1.7 290
-
 benzoyl phosphate at 40°C and pH 5.3 Pyrococcus horikoshii
3.6.1.7 290
-
 benzoyl phosphate 40°C, pH 5.3 Pyrococcus horikoshii
3.6.1.7 467
-
 benzoyl phosphate at 45°C and pH 5.3 Pyrococcus horikoshii
3.6.1.7 467
-
 benzoyl phosphate 45°C, pH 5.3 Pyrococcus horikoshii
3.6.1.7 10000
-
 benzoyl phosphate at 98°C and pH 5.3, estimation by linear extrapolation Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 5.3
-
-
 Pyrococcus horikoshii
3.6.1.7 5.3
-
 at 25°C Pyrococcus horikoshii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.7 779
-
 benzoyl phosphate 25 °C, pH 5.3 Pyrococcus horikoshii