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Literature summary extracted from

  • Kmunicek, J.; Hynkova, K.; Jedlicka, T.; Nagata, Y.; Negri, A.; Gago, F.; Wade, R.C.; Damborsky, J.
    Quantitative analysis of substrate specificity of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 (2005), Biochemistry, 44, 3390-3401.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 0.005
-
 1-Chlorohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.01
-
 1-bromohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.01
-
 1-iodohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.015
-
 1-Chloroheptane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.019
-
 1,5-Dichloropentane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.021
-
 bromocyclohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.021
-
 1-Chlorooctane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.04
-
 1,3-dibromopropane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.043
-
 1-bromobutane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.05
-
 1-bromo-2-methylpropane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 0.079
-
 3-chloro-2-(chloromethyl)-1-propene pH 8.6, 37°C Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Sphingomonas paucimobilis
-
-
-
3.8.1.5 Sphingomonas paucimobilis UT26
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,3-dibromopropane + H2O
-
 Sphingomonas paucimobilis 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-dibromopropane + H2O
-
 Sphingomonas paucimobilis UT26 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,5-dichloropentane + H2O
-
 Sphingomonas paucimobilis 5-chloro-1-pentanol + chloride
-
 ?
3.8.1.5 1-bromo-2-methylpropane + H2O
-
 Sphingomonas paucimobilis 2-methylpropanol + bromide
-
 ?
3.8.1.5 1-bromobutane + H2O
-
 Sphingomonas paucimobilis n-butanol + bromide
-
 ?
3.8.1.5 1-bromohexane + H2O
-
 Sphingomonas paucimobilis n-hexanol + bromide
-
 ?
3.8.1.5 1-chloroheptane + H2O
-
 Sphingomonas paucimobilis heptanol + chloride
-
 ?
3.8.1.5 1-chloroheptane + H2O
-
 Sphingomonas paucimobilis UT26 heptanol + chloride
-
 ?
3.8.1.5 1-chlorohexane + H2O
-
 Sphingomonas paucimobilis hexanol + chloride
-
 ?
3.8.1.5 1-chlorohexane + H2O
-
 Sphingomonas paucimobilis UT26 hexanol + chloride
-
 ?
3.8.1.5 1-chlorooctane + H2O
-
 Sphingomonas paucimobilis octanol + chloride
-
 ?
3.8.1.5 1-chlorooctane + H2O
-
 Sphingomonas paucimobilis UT26 octanol + chloride
-
 ?
3.8.1.5 1-iodohexane + H2O
-
 Sphingomonas paucimobilis n-hexanol + iodide
-
 ?
3.8.1.5 3-chloro-2-(chloromethyl)-1-propene + H2O
-
 Sphingomonas paucimobilis 2-chloromethyl-2-propen-1-ol + Cl-
-
 ?
3.8.1.5 bromocyclohexane + H2O
-
 Sphingomonas paucimobilis cyclohexanol + bromide
-
 ?
3.8.1.5 additional information quantitative analysis of substrate specificity Sphingomonas paucimobilis ?
-
 ?
3.8.1.5 additional information quantitative analysis of substrate specificity Sphingomonas paucimobilis UT26 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.5 1.033
-
 1-Chlorohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 1.05
-
 1-bromohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 1.36
-
 bromocyclohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 1.6
-
 1-bromo-2-methylpropane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 2.33
-
 1-iodohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 2.45
-
 1,5-Dichloropentane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 2.75
-
 1-Chlorooctane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 3 6 1-Chloroheptane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 3.345
-
 1-Chloroheptane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 3.41
-
 1-bromobutane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 6.08
-
 1-Chlorohexane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 6.6
-
 1,3-dibromopropane pH 8.6, 37°C Sphingomonas paucimobilis
3.8.1.5 8.17
-
 3-chloro-2-(chloromethyl)-1-propene pH 8.6, 37°C Sphingomonas paucimobilis