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Literature summary extracted from

  • Strausberg, S.L.; Ruan, B.; Fisher, K.E.; Alexander, P.A.; Bryan, P.N.
    Directed coevolution of stability and catalytic activity in calcium-free subtilisin (2005), Biochemistry, 44, 3272-3279.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.62 additional information sequential randomization of 12 amino acid positions in calcium-free enzyme. Mutations increase the half-life of enzyme at elevated temperature by 15000fold and partially increase ratio of kcat/KM-value Bacillus amyloliquefaciens
3.4.21.62 Y217L commercial version of enzyme, i.e. SBT* Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.33
-
 succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
3.4.21.62 0.36
-
 succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
3.4.21.62 0.45
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus amyloliquefaciens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
-
 Bacillus amyloliquefaciens succinyl-L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.21.62 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Bacillus amyloliquefaciens succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?
3.4.21.62 succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
-
 Bacillus amyloliquefaciens succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 subtilisin BPN’
-
 Bacillus amyloliquefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 6.4
-
 succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
3.4.21.62 68
-
 succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
3.4.21.62 210
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens