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Literature summary extracted from

  • Penheiter, A.R.; Filoteo, A.G.; Penniston, J.T.; Caride, A.J.
    Kinetic analysis of the calmodulin-binding region of the plasma membrane calcium pump isoform 4b (2005), Biochemistry, 44, 2009-2020.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.10 Calmodulin the amino acid sequence of the calmodulin binding site is L1086RRGQILWFRGLNRIQTQIKVVKAFHSS1113, i.e. peptide 28, determination of essential residues for binding, overview, complex structure modelling, calmodulin binding mechanism, overview Homo sapiens
7.2.2.10 additional information 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length bindng site peptide 28 and truncated versions, overview, complex structure modelling, calmodulin binding mechanism, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.10 F1110A 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length binding site peptide 28 and truncated and mutated versions, overview Homo sapiens
7.2.2.10 additional information 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length binding site peptide 28 and truncated and mutated versions, overview Homo sapiens
7.2.2.10 V1107A 2-chloro-(epsilon-amino-Lys75)-[6-(4-[N,N-diethylamino]phenyl)-1,3,5-triazin]-4-yl-calmodulin-labeled calmodulin is used for determination of enzyme amino acids essential for binding, peptide mapping with full-length binding site peptide 28 and truncated and mutated versions, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.2.10 additional information
-
 additional information binding kinetics of calmodulin and labeled calmodulin to enzyme binding site-derived peptides, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.10 plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.10 Ca2+
-
 Homo sapiens
7.2.2.10 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.10 ATP + H2O + Ca2+/cis Homo sapiens the enzyme is a plasma membrane calcium pump performing Ca2+-dependent ATPase and ATP-dependent Ca2+ transport reactions ADP + phosphate + Ca2+/trans
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.10 Homo sapiens
-
 isoform 4b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.10 native PMCA4b from erythrocytes by calmodulin affiniyt chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.2.10 erythrocyte isoform 4b Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.10 ATP + H2O + Ca2+/cis Ca2+-dependent ATPase and ATP-dependent Ca2+ transport activities Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?
7.2.2.10 ATP + H2O + Ca2+/cis the enzyme is a plasma membrane calcium pump performing Ca2+-dependent ATPase and ATP-dependent Ca2+ transport reactions Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.10 PCMA4b
-
 Homo sapiens
7.2.2.10 plasma membrane calcium pump
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.10 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.2.10 7.2
-
 assay at Homo sapiens