Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Borths, E.L.; Poolman, B.; Hvorup, R.N.; Locher, K.P.; Rees, D.C.
    In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptake (2005), Biochemistry, 44, 16301-16309.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.8 periplasmic binding protein BtuF 0.02 mmol, 16% increase in basal activity in lauryl dimethylamine-N-oxide, 50% increase in activity in the presence of vitamin B12 in TX-100 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.8 expressed in Escherichia coli strain BL21 (DE3) Escherichia coli

General Stability

EC Number General Stability Organism
7.6.2.8 BtuCD-F complex is stable in lauryl dimethylamine-N-oxide, dodecyl maltoside and Triton X-100 when storing the protein for several days at either 4°C or room temperature Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.8 periplasmic binding protein BtuF 4fold decrease of activity in n-alkyl-phosphocholine Escherichia coli
7.6.2.8 sodium ortho-vanadate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.8 cytoplasm
-
 Escherichia coli 5737
-
7.6.2.8 periplasm
-
 Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.8 NiNTA affinity chromatography, in the absence of vitamin B12 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6.2.8 additional information
-
 vitamin B12 has little effect on the activity in proteoliposomes, as well as in the presence of lauryl dimethylamine-N-oxide, dodecyl maltoside and Triton X-100 Escherichia coli
7.6.2.8 0.18
-
 in the absence of Btu-F and vitamin B12 in proteoliposomes Escherichia coli
7.6.2.8 0.33
-
 in the presence of Fos-choline Escherichia coli
7.6.2.8 0.44
-
 in the presence of 0.02 mmol BtuF in proteoliposomes Escherichia coli
7.6.2.8 0.98
-
 in the presence of lauryl dimethylamine-N-oxide Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.8 ATP + H2O + vitamin B12/out
-
 Escherichia coli ADP + phosphate + vitamin B12/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.8 BtuCD
-
 Escherichia coli
7.6.2.8 BtuCD-F complex of BtuCD and BtuF Escherichia coli