Literature summary extracted from

Perlow-Poehnelt, R.A.; Zharkov, D.O.; Grollman, A.P.; Broyde, S.
Substrate discrimination by formamidopyrimidine-DNA glycosylase: distinguishing interactions within the active site (2004), Biochemistry, 43, 16092-16105.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.2.23	E173Q	no enzymic activity, E173 may play a crucial role in forming the active site pocket	Escherichia coli
3.2.2.23	E2Q	no enzymic activity, interactions with G167 and Y170 are interupted	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.2.23	Escherichia coli	P05523	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2.2.23	additional information	-	molecular modeling and molecular dynamics simulation to interprete substrate discrimination in the active site	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.2.23	DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O	-	Escherichia coli	DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine	-	?
3.2.2.23	DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O	-	Escherichia coli	DNA + 4,6-diamino-5-formamidopyrimidine	-	?
3.2.2.23	DNA containing 8-oxo-7,8-dihydroguanine residues + H2O	-	Escherichia coli	DNA + 8-oxo-7,8-dihydroguanine	-	?

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Release 2023.1 (January 2023)