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Literature summary extracted from

  • Kageyama, T.
    Role of S1 loop residues in the substrate specificities of pepsin A and chymosin (2004), Biochemistry, 43, 15122-15130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.1
-
 Homo sapiens
3.4.23.4 expression in Saccharomyces cerevisiae strain INVSC1 Callithrix jacchus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.1 L291S replacement of a pepsin A-specific residue by a chymosin-specific one. Decrease in hydrolysis of peptides with hydrophobic/aromatic residues at P’1 site, increase in hydrolysis of peptides with charged residues at P’1 Homo sapiens
3.4.23.1 L291S/L298Q replacement of a pepsin A-specific residue by a chymosin-specific one. Decrease in hydrolysis of peptides with hydrophobic/aromatic residues at P’1 site, increase in hydrolysis of peptides with charged residues at P’1 Homo sapiens
3.4.23.1 L298Q replacement of a pepsin A-specific residue by a chymosin-specific one. Decrease in hydrolysis of peptides with hydrophobic/aromatic residues at P’1 site, increase in hydrolysis of peptides with charged residues at P’1 Homo sapiens
3.4.23.1 M289D replacement of a pepsin A-specific residue by a chymosin-specific one. Decrease in hydrolysis of peptides with hydrophobic/aromatic residues at P’1 site, increase in hydrolysis of peptides with charged residues at P’1 Homo sapiens
3.4.23.4 D289M/Q298L decreased hydrolytic activity Callithrix jacchus
3.4.23.4 additional information Asp289Met mutant, Gln298Leu mutant, hydrolytic activity toward peptides having Lys at P'1 decreases significantly, mainly because of a decrease in the turnover values Callithrix jacchus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.4 0.016
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 0.024
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 0.028
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 0.029
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 0.033
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 0.046
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 0.054
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 0.054
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 0.059
-
 NT/NMN 142-151 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus
3.4.23.4 0.062
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 0.065
-
 POm C165-174 hydrolysis of the Glu171 variant by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 0.072
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 0.083
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by wild type chymosin Callithrix jacchus
3.4.23.4 0.087
-
 NT/NMN 142-151 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 0.095
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 0.105
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by wild type chymosin Callithrix jacchus
3.4.23.4 0.111
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by wild type chymosin Callithrix jacchus
3.4.23.4 0.114
-
 POm C165-174 hydrolysis of the Glu171 variant by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 0.229
-
 POm C165-174 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 0.25
-
 POm C165-174 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 0.288
-
 POm C165-174 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 0.304
-
 POm C165-174 hydrolysis of the Glu171 variant by wild type chymosin Callithrix jacchus
3.4.23.4 0.375
-
 POm C165-174 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus
3.4.23.4 0.463
-
 POm C165-174 hydrolysis of the Glu171 variant by Gln298Leu mutant chymosin Callithrix jacchus

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.1 Homo sapiens
-
-
-
3.4.23.4 Callithrix jacchus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.4
-
 Callithrix jacchus

Storage Stability

EC Number Storage Stability Organism
3.4.23.4 -20°C, Tris-HCl buffer, pH 8.0, glycerol Callithrix jacchus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.1 additional information preference for hydrophobic and aromatic residues at P’1 site of substrate Homo sapiens ?
-
 ?
3.4.23.4 basic FGF 110-118 + H2O parent peptide and Leu115 and Lys115 variants respectively as substrate Callithrix jacchus ?
-
 ?
3.4.23.4 dynorphin A 1-7e + H2O Ala3Phe7 and ILe3Lys5Phe7 variants respectively as substrate Callithrix jacchus ?
-
 ?
3.4.23.4 NT/NMN 142-151 + H2O parent peptide, Glu148 and Phe148 variants respectively as substrate Callithrix jacchus ?
-
 ?
3.4.23.4 POm C165-174 + H2O parent peptide and Glu171 variant respectively as substrate Callithrix jacchus ?
-
 ?
3.4.23.4 Substance P + H2O parent peptide and Lys8 variant respectively as substrate Callithrix jacchus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.4 2 8 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by wild type chymosin Callithrix jacchus
3.4.23.4 12
-
 POm C165-174 hydrolysis of the Glu171 variant by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 12
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 14
-
 POm C165-174 hydrolysis of the Glu171 variant by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 16
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 17
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 17
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 22
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 27
-
 NT/NMN 142-151 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 30
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 32
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 35
-
 NT/NMN 142-151 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 39
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage L147-F148) by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 41
-
 NT/NMN 142-151 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus
3.4.23.4 42
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 43
-
 POm C165-174 hydrolysis of the parent peptide by D289M/Q298L mutant chymosin Callithrix jacchus
3.4.23.4 46
-
 NT/NMN 142-151 hydrolysis of the Glu148 variant by wild type chymosin Callithrix jacchus
3.4.23.4 49
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 52
-
 POm C165-174 hydrolysis of the Glu171 variant by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 63
-
 POm C165-174 hydrolysis of the Glu171 variant by wild type chymosin Callithrix jacchus
3.4.23.4 90
-
 POm C165-174 hydrolysis of the parent peptide by Gln298Leu mutant chymosin Callithrix jacchus
3.4.23.4 101
-
 POm C165-174 hydrolysis of the parent peptide by Asp289Met mutant chymosin Callithrix jacchus
3.4.23.4 191
-
 NT/NMN 142-151 hydrolysis of the Phe148 variant (cleavage F147-R148) by wild type chymosin Callithrix jacchus
3.4.23.4 219
-
 POm C165-174 hydrolysis of the parent peptide by wild type chymosin Callithrix jacchus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.4 4
-
-
 Callithrix jacchus