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Literature summary extracted from

  • Chang, Q.L.; Gummadi, S.N.; Menon, A.K.
    Chemical modification identifies two populations of glycerophospholipid flippase in rat liver ER (2004), Biochemistry, 43, 10710-10718.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.1 diethyldicarbonate rat liver contains two populations, DEPC-sensitive and DEPC-insensitive, of flippases, which can be in a different of DEPC sensitivity or be two different proteins Rattus norvegicus
7.6.2.1 NEM rat liver contains two populations of, NEM-sensitive and NEM-insensitive, flippases, which can be in a different of NEM sensitivity or be two different proteins Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.1 endoplasmic reticulum membrane
-
 Rattus norvegicus 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.1 additional information Rattus norvegicus the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process ?
-
 ?
7.6.2.1 phospholipid/in Rattus norvegicus
-
 phospholipid/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.1 native enzyme from liver endoplasmic reticulum Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
7.6.2.1 reconstitution of the purified native enzyme in proteoliposomes using phospholipids and Triton X-100 at pH 7.5 and 22°C Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.6.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6.2.1 additional information
-
 aminophospholipid translocase assay using dithionite reduction by 4-nitrobenzo-2-oxa-1,3-diazole-labeled phospholipids, dithionate does not permeate membranes Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.1 additional information the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process Rattus norvegicus ?
-
 ?
7.6.2.1 phosphatidylcholine/in proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum Rattus norvegicus phosphatidylcholine/out
-
 ?
7.6.2.1 phosphatidylethanolamine/in proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum Rattus norvegicus phosphatidylethanolemine/out
-
 ?
7.6.2.1 phosphatidylserine/in proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum Rattus norvegicus phosphatidylserine/out
-
 ?
7.6.2.1 phospholipid/in
-
 Rattus norvegicus phospholipid/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.1 glycerophospholipid flippase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.6.2.1 22
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6.2.1 7.5
-
 assay at Rattus norvegicus