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Literature summary extracted from

  • Steiger, M.A.; Kierzek, R.; Turner, D.H.; Phizicky, E.M.
    Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog (2001), Biochemistry, 40, 14098-14105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.160 expression in Escherichia coli BL21(DE3) Escherichia coli
2.7.1.160 overexpression in Escherichia coli BL21(DE3) Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.160 ApGpApUpUpUpApC oligonucleotide, which lacks secondary structure and has no terminal phosphate Saccharomyces cerevisiae
2.7.1.160 [pre-tRNA] intron-containing yeast [pre-tRNA], which has the same structure as mature yeast [tRNA], except in the region of the intron Saccharomyces cerevisiae
2.7.1.160 [tRNA] mature Escherichia coli [tRNA], has canonical tRNA structue Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.160 0.00000035
-
 2'-phospho-[ligated tRNA]
-
 Saccharomyces cerevisiae
2.7.1.160 0.0000017
-
 18-mer 2' phosphorylated RNA pACAAGACUCUAA-(2’-phosphate)AUCUUG, 30°C Saccharomyces cerevisiae
2.7.1.160 0.000004
-
 2'-phospho-[ligated tRNA]
-
 Escherichia coli
2.7.1.160 0.00002
-
 8-mer 2' phosphorylated RNA pGUAA-(2’-phosphate)AUCU, 30°C Saccharomyces cerevisiae
2.7.1.160 0.00015
-
 18-mer 2' phosphorylated RNA 37°C Escherichia coli
2.7.1.160 0.00015
-
 3-mer 2' phosphorylated RNA pApA-(2’-phosphate)pA, 30°C Saccharomyces cerevisiae
2.7.1.160 0.00025
-
 8-mer 2' phosphorylated RNA 37°C Escherichia coli
2.7.1.160 0.000325
-
 3-mer 2' phosphorylated RNA pUpU-(2’-phosphate)pUs, 30°C Saccharomyces cerevisiae
2.7.1.160 0.00048
-
 3-mer 2' phosphorylated RNA pApU-(2’-phosphate)pA, 30°C Saccharomyces cerevisiae
2.7.1.160 0.00065
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate)p, 30°C Saccharomyces cerevisiae
2.7.1.160 0.0007
-
 3-mer 2' phosphorylated RNA pApA-(2’-phosphate)pA, 37°C Escherichia coli
2.7.1.160 0.0013
-
 3-mer 2' phosphorylated RNA pAAA-(2’-phosphate)p, 37°C Escherichia coli
2.7.1.160 0.0018
-
 3-mer 2' phosphorylated RNA pUpU-(2’-phosphate)pU, 37°C Escherichia coli
2.7.1.160 0.003
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate)pOCH3, 30°C Saccharomyces cerevisiae
2.7.1.160 0.0038
-
 3-mer 2' phosphorylated RNA pAAA-(2’-phosphate)pOCH3, 37°C Escherichia coli
2.7.1.160 0.04
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate), 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.160 30000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.160 Escherichia coli
-
-
-
2.7.1.160 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.160 KptA-His6 10% purified by sonication, immobilized metal ion chromatography, centrifugation Escherichia coli
2.7.1.160 Tpt1-His6 50% purified by sonication, immobilized metal ion chromatography, centrifugation Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.160 additional information
-
 200000000 units/mg for recombinant Tpt1 produced in Escherichia coli and 140000000 units/mg for Tpt1 purified from yeast Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.160 -20°C, Tris buffer, pH 7.5, 50% glycerol Escherichia coli
2.7.1.160 -20°C, Tris buffer, pH 7.5, 50% glycerol Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.160 18-mer 2' phosphorylated RNA
-
 Escherichia coli ?
-
 ?
2.7.1.160 18-mer 2' phosphorylated RNA pACAAGACUCUAA-(2’-phosphate)AUCUUG Saccharomyces cerevisiae ?
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA]
-
 Escherichia coli ?
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA]
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+
-
 Escherichia coli mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide
-
 ?
2.7.1.160 3-mer 2' phosphorylated RNA pApA-(2’-phosphate)pA, pApU-(2’-phosphate)pA, pUpU-(2’-phosphate)pU, pApApA-(2’-phosphate),pApApA-(2’-phosphate)p, pApApA-(2’-phosphate)1OCH3 Saccharomyces cerevisiae ?
-
 ?
2.7.1.160 3-mer 2' phosphorylated RNA pApa-(2’-phosphate)pA, pUpU-(2’-phosphate)pU, pAAA-(2’-phosphate)p and pAAA-(2’-phosphate)pOCH3 Escherichia coli ?
-
 ?
2.7.1.160 8-mer 2' phosphorylated RNA
-
 Escherichia coli ?
-
 ?
2.7.1.160 8-mer 2' phosphorylated RNA pGUAA-(2’-phosphate)AUCU Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.160 KptA
-
 Escherichia coli
2.7.1.160 Tpt1
-
 Saccharomyces cerevisiae
2.7.1.160 yeast 2'-phosphotransferase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.160 additional information
-
 8-mer 2' phosphorylated RNA 37°C, vmax = 170 fmol/min/ng Escherichia coli
2.7.1.160 additional information
-
 18-mer 2' phosphorylated RNA 37°C, vmax = 99 fmol/min/ng Escherichia coli
2.7.1.160 additional information
-
 3-mer 2' phosphorylated RNA pAAA-(2’-phosphate)p, 37°C, vmax = 1.4 fmol/min/ng Escherichia coli
2.7.1.160 additional information
-
 3-mer 2' phosphorylated RNA pAAA-(2’-phosphate)pOCH3, 37°C, vmax = 45 fmol/min/ng Escherichia coli
2.7.1.160 additional information
-
 3-mer 2' phosphorylated RNA pApA-(2’-phosphate)pA, 37°C, vmax = 280 fmol/min/ng Escherichia coli
2.7.1.160 additional information
-
 3-mer 2' phosphorylated RNA pUpU-(2’-phosphate)pU, 37°C, vmax = 1030 fmol/min/ng Escherichia coli
2.7.1.160 additional information
-
 [ligated tRNA] vmax = 0.26 fmol/min/ng Escherichia coli
2.7.1.160 0.0016
-
 2'-phospho-[ligated tRNA]
-
 Saccharomyces cerevisiae
2.7.1.160 0.0057
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate), 30°C Saccharomyces cerevisiae
2.7.1.160 0.04
-
 18-mer 2' phosphorylated RNA pACAAGACUCUAA-(2’-phosphate)AUCUUG, 30°C Saccharomyces cerevisiae
2.7.1.160 0.147
-
 3-mer 2' phosphorylated RNA pUpU-(2’-phosphate)pU, 30°C Saccharomyces cerevisiae
2.7.1.160 0.15
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate)p, 30°C Saccharomyces cerevisiae
2.7.1.160 0.317
-
 8-mer 2' phosphorylated RNA pGUAA-(2’-phosphate)AUCU, 30°C Saccharomyces cerevisiae
2.7.1.160 0.45
-
 3-mer 2' phosphorylated RNA pApU-(2’-phosphate)pA, 30°C Saccharomyces cerevisiae
2.7.1.160 0.73
-
 3-mer 2' phosphorylated RNA pApA-(2’-phosphate)pA, 30°C Saccharomyces cerevisiae
2.7.1.160 1.173
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate)pOCH3, 30°C Saccharomyces cerevisiae
2.7.1.160 6.08
-
 3-mer 2' phosphorylated RNA pApApA-(2’-phosphate)pOCH3, 30°C Saccharomyces cerevisiae