Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lehoux, I.E.; Mitra, B.
    (S)-Mandelate dehydrogenase from Pseudomonas putida: mutations of the catalytic base histidine-274 and chemical rescue of activity (1999), Biochemistry, 38, 9948-9955.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.31 H274A inactive mutant, activity can partially be restored by the addition of exogenous imidazole Pseudomonas putida
1.1.99.31 H274D inactive mutant Pseudomonas putida
1.1.99.31 H274G inactive mutant, activity can be restored by the addition of exogenous imidazole Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 13
-
 (S)-Mandelate mutant enzyme H274G, in presence of 20 mM imidazole Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.31 mutant enzymes H274G, H274A and H274N Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (R,S)-p-chloromandelate + acceptor
-
 Pseudomonas putida ?
-
 ?
1.1.99.31 (S)-mandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.31 0.03
-
 (R,S)-p-chloromandelate mutant enzyme H274G, in presence of 20 mM imidazole Pseudomonas putida
1.1.99.31 0.09
-
 (S)-Mandelate mutant enzyme H274G, in presence of 20 mM imidazole Pseudomonas putida