BRENDA - Enzyme Database show

Modifications of deoxycytidine kinase and deaminase activities by docosahexaenoic acid in normal and transformed rat fibroblasts

Cha, M.C.; Meckling-Gill, K.A.; Biochem. Pharmacol. 63, 717-723 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
increases the enzyme activity in vivo in normal Rat-2 fibroblasts, but decreases it in transformed NM-16 fibroblasts
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
decreases the enzyme activity in vivo in transformed NM-16 fibroblasts, but increases it in normal Rat-2 fibroblasts
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.347
-
deoxycytidine
pH 8.1, 37°C, Rat-2 fibroblasts in presence of 0.02 mM docosahexanoic acid
Rattus norvegicus
3.5.4.12
0.349
-
deoxycytidine
pH 8.1, 37°C, Rat-2 fibroblasts
Rattus norvegicus
3.5.4.12
0.462
-
deoxycytidine
pH 8.1, 37°C, NM-16 fibroblasts
Rattus norvegicus
3.5.4.12
0.467
-
deoxycytidine
pH 8.1, 37°C, NM-16 fibroblasts in presence of 0.02 mM docosahexanoic acid
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
deoxycytidine + H2O
Rattus norvegicus
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
deoxyuridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
fibroblast
the enzyme activity is higher in normal Rat-2 fibroblast compared to transformed NM-16 cells
Rattus norvegicus
-
3.5.4.12
NW-16 cell
transformed fibroblast cell line
Rattus norvegicus
-
3.5.4.12
Rat-2 cell
fibroblast cell line
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
deoxycytidine + H2O
-
667541
Rattus norvegicus
deoxyuridine + NH3
-
-
-
?
3.5.4.12
deoxycytidine + H2O
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
667541
Rattus norvegicus
deoxyuridine + NH3
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8.1
-
assay at
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
increases the enzyme activity in vivo in normal Rat-2 fibroblasts, but decreases it in transformed NM-16 fibroblasts
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
decreases the enzyme activity in vivo in transformed NM-16 fibroblasts, but increases it in normal Rat-2 fibroblasts
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.347
-
deoxycytidine
pH 8.1, 37°C, Rat-2 fibroblasts in presence of 0.02 mM docosahexanoic acid
Rattus norvegicus
3.5.4.12
0.349
-
deoxycytidine
pH 8.1, 37°C, Rat-2 fibroblasts
Rattus norvegicus
3.5.4.12
0.462
-
deoxycytidine
pH 8.1, 37°C, NM-16 fibroblasts
Rattus norvegicus
3.5.4.12
0.467
-
deoxycytidine
pH 8.1, 37°C, NM-16 fibroblasts in presence of 0.02 mM docosahexanoic acid
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
deoxycytidine + H2O
Rattus norvegicus
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
deoxyuridine + NH3
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
fibroblast
the enzyme activity is higher in normal Rat-2 fibroblast compared to transformed NM-16 cells
Rattus norvegicus
-
3.5.4.12
NW-16 cell
transformed fibroblast cell line
Rattus norvegicus
-
3.5.4.12
Rat-2 cell
fibroblast cell line
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
deoxycytidine + H2O
-
667541
Rattus norvegicus
deoxyuridine + NH3
-
-
-
?
3.5.4.12
deoxycytidine + H2O
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
667541
Rattus norvegicus
deoxyuridine + NH3
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8.1
-
assay at
Rattus norvegicus