EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.105 | - |
Homo sapiens |
1.1.1.300 | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.105 | 0.0011 | - |
all-trans-retinol | 37°C | Homo sapiens | |
1.1.1.300 | 0.18 | - |
all-trans-retinol | 37°C | Homo sapiens | |
1.1.1.300 | 0.3 | - |
all-trans-retinal | 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.300 | membrane | microsomal membrane | Homo sapiens | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.105 | all-trans-retinol + NAD+ | Homo sapiens | RoDH-4 is capable of contributing to the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in the cellular context | all-trans-retinal + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.105 | Homo sapiens | O75452 | - |
- |
1.1.1.300 | Homo sapiens | Q8TC12 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.105 | preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel | Homo sapiens |
1.1.1.300 | purified to homogeneity | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.105 | all-trans-retinol + NAD+ | RoDH-4 is capable of contributing to the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in the cellular context | Homo sapiens | all-trans-retinal + NADH | - |
? | |
1.1.1.105 | all-trans-retinol + NAD+ | - |
Homo sapiens | all-trans-retinal + NADH + H+ | - |
? | |
1.1.1.105 | additional information | the enzymes utilizes retinol bound to cellular retinol binding protein type I at a much lower rate than free retinol | Homo sapiens | ? | - |
? | |
1.1.1.300 | all-trans-retinol + NADP+ | more efficient in the reductive direction | Homo sapiens | all-trans-retinal + NADPH + H+ | - |
r | |
1.1.1.300 | additional information | the enzymes utilizes retinol bound to cellular retinol binding protein type I at a much lower rate than free retinol | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.105 | RoDH-4 | - |
Homo sapiens |
1.1.1.300 | RDH11 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.105 | 0.02 | - |
all-trans-retinol | 37°C | Homo sapiens | |
1.1.1.300 | 0.00012 | - |
all-trans-retinal | 37°C | Homo sapiens | |
1.1.1.300 | 0.0006 | - |
all-trans-retinol | 37°C | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.105 | NAD+ | preferred cofactor | Homo sapiens | |
1.1.1.300 | NADP+ | preferred cofactor | Homo sapiens | |
1.1.1.300 | NADPH | preferred cofactor | Homo sapiens |