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Literature summary extracted from
- A Toxoplasma gondii phosphoinositide phospholipase C (TgPI-PLC) with high affinity for phosphatidylinositol (2006), Biochem. J., 394, 417-425.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.11 | expression in Escherichia coli BL21 | Toxoplasma gondii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.11 | Ca2+ | when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 1 µM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol | Toxoplasma gondii |  |
| 3.1.4.11 | Mg2+ | hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
| 3.1.4.11 | neomycin | hydrolysis of phosphatidylinositol and 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
| 3.1.4.11 | substance 48/80 | inhibition is more pronounced with phosphatidylinositol as substrate than with 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.11 | 0.079 | - |
phosphatidylinositol | recombinant enzyme | Toxoplasma gondii | |
| 3.1.4.11 | 0.13 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | recombinant enzyme | Toxoplasma gondii | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.11 | plasma membrane | extracellular phase of plasma membrane | Toxoplasma gondii | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.11 | Ca2+ | when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 0.001 mM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
| 3.1.4.11 | Mg2+ | slight stimulation of hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.11 | 121000 | - |
calculated from sequence | Toxoplasma gondii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.11 | Toxoplasma gondii | Q3ZTU7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.11 | - |
Toxoplasma gondii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.11 | tachyzoite | - |
Toxoplasma gondii | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.11 | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Toxoplasma gondii | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| 3.1.4.11 | phosphatidylinositol + H2O | - |
Toxoplasma gondii | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.11 | TgPI-PLC | - |
Toxoplasma gondii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.11 | 20 | - |
hydrolysis of phosphatidylinositol | Toxoplasma gondii |
| 3.1.4.11 | 30 | - |
hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.11 | 5 | 30 | 5°C: about 50% of maximal activity, 30°C: about 45% of maximal activity, hydrolysis of phosphatidylinositol | Toxoplasma gondii |
| 3.1.4.11 | 15 | 37 | 15°C: about 75% of maximal activity, 37°C: about 80% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.11 | 7.2 | 7.5 | hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
| 3.1.4.11 | 8 | - |
hydrolysis of phosphatidylinositol | Toxoplasma gondii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.11 | 7.1 | 7.6 | pH 7.1: about 85% of maximal activity, pH 7.6: about 65% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
| 3.1.4.11 | 7.1 | 9.2 | pH 7.1: about 90% of maximal activity, pH 9.2: about 85% of maximal activity, hydrolysis of phosphatidylinositol | Toxoplasma gondii |
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Release 2023.1 (January 2023)
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