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Literature summary extracted from

  • Vishu Kumar, A.B.; Varadaraj, M.C.; Gowda, L.R.; Tharanathan, R.N.
    Characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and pronase, and their bactericidal action against Bacillus cereus and Escherichia coli (2005), Biochem. J., 391, 167-175.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.2 extracellular latex Carica papaya
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.2 chitosan + H2O Carica papaya depolymerization, the enzyme inhibits the growth of bacteria such as Bacillus cereus strain F4810, Bacillus licheniformis, and Escherichia coli strain D21, mechanism of bactericidal action of the chito-oligomeric-monomeric mixture, overview low-molecular mass chitosan + chito-oligomeric-monomeric mixture
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.2 further purification of the commercial enzyme preparation from latex by gel filtration, to homogeneity Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 commercial preparation from latex Carica papaya
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3.4.22.2 latex
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 Carica papaya
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.2 additional information
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 growth inhibition of Bacillus cereus strain F4810 and Escherichia coli strain D21 via the chitosanolytic activity of the enzyme Carica papaya
3.4.22.2 1.78
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 purified enzyme, substrate chitosan Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 chitosan + H2O depolymerization Carica papaya low-molecular mass chitosan + chito-oligomeric-monomeric mixture product analysis by FTIR and NMR spectroscopy, overview ?
3.4.22.2 chitosan + H2O depolymerization, the enzyme inhibits the growth of bacteria such as Bacillus cereus strain F4810, Bacillus licheniformis, and Escherichia coli strain D21, mechanism of bactericidal action of the chito-oligomeric-monomeric mixture, overview Carica papaya low-molecular mass chitosan + chito-oligomeric-monomeric mixture
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 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 37
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 depolymerization of chitosan Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 3.5
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 depolymerization of chitosan Carica papaya