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Literature summary extracted from

  • Wladyka, B.; Puzia, K.; Dubin, A.
    Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli (2005), Biochem. J., 385, 181-187.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.48 co-expression of His-tagged staphopain A and its inhibitor staphostatin A in Escherichia coli strain BL21(DE3) in inclusion bodies, recombinant enzyme is toxic for Escherichia coli cells, therefore a co-expression with the enzyme inhibitor is required for recombinant enzyme production, method optimization Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.48 additional information construction of an inactive mutant, active site mutation of staphopain A results in abrogation of production of both co-expressed proteins Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.48 recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by refolding and nickel affinity chromatography, method optimization Staphylococcus aureus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.48 recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by treatment with 6 M guanidinium hydrochloride, and dilution in buffer, at pH 6.0, with 40% glycerol content or with arginine and Tween 20 supplementation, method optimization Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.48 staphopain A
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.48 37
-
 assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.48 7
-
 assay at Staphylococcus aureus