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Literature summary extracted from
- Augmented intrinsic activity of Factor VIIa by replacement of residues 305, 314, 337 and 374: evidence of two unique mutational mechanisms of activity enhancement (2004), Biochem. J., 379, 497-503.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.21 | expressed in baby hamster kidney cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.21 | antithrombin | 0.1 mg/ml in the presence of 1 unit/ml heparin, less than 10% residual activity after 15min incubation with enzyme FVIIa-sTF | Homo sapiens | |
| 3.4.21.21 | p-aminobenzamidine | - |
Homo sapiens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.21 | 0.0018 | - |
Factor X | mutant FVIIaIIa | Homo sapiens | |
| 3.4.21.21 | 0.0021 | - |
Factor X | mutant FVIIaIIa-sTF | Homo sapiens | |
| 3.4.21.21 | 0.0021 | - |
Factor X | mutant FVIIaVEAY-sTF | Homo sapiens | |
| 3.4.21.21 | 0.0027 | - |
Factor X | mutant FVIIaVEAY | Homo sapiens | |
| 3.4.21.21 | 0.003 | - |
Factor X | wild type FVIIa-sTF | Homo sapiens | |
| 3.4.21.21 | 0.0044 | - |
Factor X | wild type FVIIa | Homo sapiens | |
| 3.4.21.21 | 0.85 | - |
D-Ile-Pro-Arg-p-nitroanilide | mutant FVIIaVEAY-sTF | Homo sapiens | |
| 3.4.21.21 | 1.6 | - |
D-Ile-Pro-Arg-p-nitroanilide | wild type FVIIa-sTF | Homo sapiens | |
| 3.4.21.21 | 1.7 | - |
D-Ile-Pro-Arg-p-nitroanilide | mutant FVIIaIIa-sTF | Homo sapiens | |
| 3.4.21.21 | 1.9 | - |
D-Ile-Pro-Arg-p-nitroanilide | mutant FVIIaVEAY | Homo sapiens | |
| 3.4.21.21 | 6.7 | - |
D-Ile-Pro-Arg-p-nitroanilide | mutant FVIIaIIa | Homo sapiens | |
| 3.4.21.21 | 10.4 | - |
D-Ile-Pro-Arg-p-nitroanilide | wild type FVIIa | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.21 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.21 | method not mentioned | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.21 | benzyloxycarbonyl-D-arginyl-D-glycyl-D-arginine-p-nitroanilide + H2O | - |
Homo sapiens | p-nitroaniline + benzyloxycarbonyl-D-arginyl-D-glycyl-D-arginine | - |
? | |
| 3.4.21.21 | D-Ile-Pro-Arg-p-nitroanilide + H2O | - |
Homo sapiens | p-nitroaniline + D-Ile-Pro-Arg | - |
? | |
| 3.4.21.21 | D-Phe-pipecolyl-Arg-p-nitroanilide + H2O | - |
Homo sapiens | D-Phe-pipecolyl-Arg + p-nitroaniline | - |
? | |
| 3.4.21.21 | factor X + H2O | - |
Homo sapiens | factor Xa | - |
? | |
| 3.4.21.21 | pyroGlu-Pro-Arg-p-nitroanilide + H2O | - |
Homo sapiens | p-nitroaniline + pyroGlu-Pro-Arg | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.21 | FVIIa | - |
Homo sapiens |
| 3.4.21.21 | FVIIa-sTF | FVIIa in complex with soluble tissue factor | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.21 | 0.00015 | - |
Factor X | wild type FVIIa | Homo sapiens | |
| 3.4.21.21 | 0.0012 | - |
Factor X | mutant FVIIaVEAY | Homo sapiens | |
| 3.4.21.21 | 0.0053 | - |
Factor X | mutant FVIIaIIa | Homo sapiens | |
| 3.4.21.21 | 0.007 | - |
Factor X | mutant FVIIaVEAY-sTF | Homo sapiens | |
| 3.4.21.21 | 0.0077 | - |
Factor X | wild type FVIIa-sTF | Homo sapiens | |
| 3.4.21.21 | 0.024 | - |
Factor X | mutant FVIIaIIa-sTF | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.21 | additional information | - |
additional information | because of competitive binding of p-aminobenzamidine and D-Ile-Pro-Arg-p-nitroanilide, the apparent Ki values were calculated from the p-aminobenzamidine IC50, taking into account the concentration of D-Ile-Pro-Arg-p-nitroanilide ([S]) and the Km for this substrate using Ki =IC50/(1 + [S]/Km) | Homo sapiens | |
| 3.4.21.21 | 0.038 | - |
p-aminobenzamidine | mutant FVIIaVEAY-sTF, 210 nM | Homo sapiens | |
| 3.4.21.21 | 0.05 | - |
p-aminobenzamidine | mutant FVIIaIIa-sTF, 210 nM | Homo sapiens | |
| 3.4.21.21 | 0.077 | - |
p-aminobenzamidine | wild type FVIIa-sTF, 210 nM | Homo sapiens | |
| 3.4.21.21 | 0.177 | - |
p-aminobenzamidine | mutant FVIIaVEAY, 10 nM | Homo sapiens | |
| 3.4.21.21 | 0.226 | - |
p-aminobenzamidine | mutant FVIIaIIa, 25 nM | Homo sapiens | |
| 3.4.21.21 | 1.41 | - |
p-aminobenzamidine | wild type FVIIa, 100 nM | Homo sapiens | |
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