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Literature summary extracted from

  • Povelainen, M.; Eneyaskaya, E.V.; Kulminskaya, A.A.; Ivanen, D.R.; Kalkinnen, N.; Neustroev, K.N.; Miasnikov, A.N.
    Biochemical and genetic characterization of a novel enzyme of pentitol metabolism: D-arabitol-phosphate dehydrogenase (2003), Biochem. J., 371, 191-197.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.301
-
 Enterococcus avium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.301 EDTA xylulose 5-phosphate does not protect the enzyme from EDTA inactivation. Addition of Mn2+ at concentrations of up to 2 mM results in complete reactivation of APDH Enterococcus avium
1.1.1.301 Hg2+ 2 mM, complete inactivation Enterococcus avium
1.1.1.301 PHMB 2 mM, complete inactivation Enterococcus avium
1.1.1.301 Zn2+ 2 mM, complete inactivation Enterococcus avium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.301 0.021
-
 NADH 20°C, pH 7.2, cosubstrate: D-xylulose 5-phosphate Enterococcus avium
1.1.1.301 0.23
-
 D-xylulose 5-phosphate 20°C, pH 7.2, cofactor: NADH Enterococcus avium
1.1.1.301 0.24
-
 NADPH 20°C, pH 7.2, cosubstrate: D-xylulose 5-phosphate Enterococcus avium
1.1.1.301 0.63
-
 D-arabitol 5-phosphate 20°C, pH 8.5, cofactor: NAD+ Enterococcus avium
1.1.1.301 0.65
-
 D-xylulose 5-phosphate 20°C, pH 7.2, cofactor NADPH Enterococcus avium
1.1.1.301 0.71
-
 NAD+ 20°C, pH 8.5: cosubstrate: D-arabitol 5-phosphate Enterococcus avium
1.1.1.301 0.8
-
 NAD+ 20°C, pH 8.5, cosubstrate: D-arabitol 1-phosphate Enterococcus avium
1.1.1.301 2.7
-
 NADP+ 20°C, pH 8.5, cosubstrate: D-arabitol 1-phosphate Enterococcus avium
1.1.1.301 2.9
-
 D-arabitol 1-phosphate 20°C, pH 8.5, cofactor: NAD+ Enterococcus avium
1.1.1.301 3.6
-
 D-arabitol 1-phosphate 20°C, pH 8.5, cofactor: NADP+ Enterococcus avium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.301 Mn2+ required. Pure APDH contains 4.05 ions of Mn2+ Enterococcus avium
1.1.1.301 additional information no requirement for Zn2+ Enterococcus avium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.301 41000
-
 4 * 41000, SDS-PAGE Enterococcus avium
1.1.1.301 160000
-
 non-denaturing PAGE Enterococcus avium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.301 additional information Enterococcus avium participates in arabitol catabolism via the arabitol phosphate route ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.301 Enterococcus avium Q8KQL2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.301
-
 Enterococcus avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.301 D-arabitol 1-phosphate + NAD+
-
 Enterococcus avium D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.301 D-arabitol 1-phosphate + NADP+
-
 Enterococcus avium D-xylulose 5-phosphate + NADPH + H+
-
 r
1.1.1.301 D-arabitol 5-phosphate + NAD+
-
 Enterococcus avium D-ribulose 5-phosphate + NADH + H+
-
 ?
1.1.1.301 D-arabitol 5-phosphate + NADP+
-
 Enterococcus avium D-ribulose 5-phosphate + NADPH + H+
-
 ?
1.1.1.301 additional information participates in arabitol catabolism via the arabitol phosphate route Enterococcus avium ?
-
 ?
1.1.1.301 additional information no activity with: xylitol 5-phosphate, D-sorbitol, D-mannitol, xylitol, erythrose 4-phosphate and ribose 5-phosphate. APDH kinetics are consistent with a ternary-complex mechanism Enterococcus avium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.301 tetramer 4 * 41000, SDS-PAGE Enterococcus avium

Synonyms

EC Number Synonyms Comment Organism
1.1.1.301 APDH
-
 Enterococcus avium
1.1.1.301 D-arabitol 1-phosphate dehydrogenase
-
 Enterococcus avium
1.1.1.301 D-arabitol 5-phosphate dehydrogenase
-
 Enterococcus avium
1.1.1.301 D-arabitol-phosphate dehydrogenase
-
 Enterococcus avium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.301 6.8 7.4 reduction of D-xylulose 5-phosphate with NADH or NADPH Enterococcus avium
1.1.1.301 8.3 8.6 oxidation of D-arabitol 1-phosphate with NAD+ or NADP+ Enterococcus avium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.301 NAD+ the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium
1.1.1.301 NADH the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium
1.1.1.301 NADP+ the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium
1.1.1.301 NADPH the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.301 Enterococcus avium isoelectric focusing
-
 6.4