EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.73 | - |
Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.76 | - |
methyl ferulate | FAEA | Aspergillus niger | |
3.1.1.73 | 1.31 | - |
methyl ferulate | FAEB | Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.73 | extracellular | - |
Aspergillus niger | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 74000 | - |
x * 74000, SDS-PAGE of intact protein, deglycosylated protein has a mass of 60000 Da | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | arabinoxylan + H2O | Aspergillus niger | from wheat | ferulic acid + ? | - |
? | |
3.1.1.73 | feruloyl polysaccharide + H2O | Aspergillus niger | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
3.1.1.73 | pectin + H2O | Aspergillus niger | from sugar beet | ferulic acid + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Aspergillus niger | O42807 | type A enzyme; feruloyl esterase type A | - |
3.1.1.73 | Aspergillus niger | Q8WZI8 | type B enzyme; feruloyl esterase type B | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.73 | glycoprotein | 18 glycosylation sides | Aspergillus niger |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | - |
Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | arabinoxylan + H2O | from wheat | Aspergillus niger | ferulic acid + ? | - |
? | |
3.1.1.73 | ethyl ferulate + H2O | hydrolyzed by FAEA and not FAEB with FAEA displaying the higher activity | Aspergillus niger | ferulic acid + ethanol | - |
? | |
3.1.1.73 | feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Aspergillus niger | ferulic acid + ? | - |
? | |
3.1.1.73 | methyl 3,5-dimethoxy-4-hydroxy-cinnamate + H2O | hydrolyzed only by FAEA and not FAEB | Aspergillus niger | 3,5-dimethoxy-4-hydroxycinnamic acid + methanol | - |
? | |
3.1.1.73 | methyl caffeate + H2O | hydrolyzed only by FAEB and not FAEA | Aspergillus niger | caffeic acid + methanol | - |
? | |
3.1.1.73 | methyl cinnamate + H2O | hydrolyzed only by FAEB and not FAEA | Aspergillus niger | cinnamic acid + methanol | - |
? | |
3.1.1.73 | methyl ferulate + H2O | hydrolyzed by FAEA and not FAEB with FAEA displaying the higher activity | Aspergillus niger | ferulic acid + methanol | - |
? | |
3.1.1.73 | additional information | no hydrolysis of methyl gallate | Aspergillus niger | ? | - |
? | |
3.1.1.73 | pectin + H2O | from sugar beet | Aspergillus niger | ferulic acid + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | ? | x * 55551.4, calculated from the deduced amino acid sequence | Aspergillus niger |
3.1.1.73 | ? | x * 74000, SDS-PAGE of intact protein, deglycosylated protein has a mass of 60000 Da | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | FAEA | - |
Aspergillus niger |
3.1.1.73 | FAEB | - |
Aspergillus niger |
3.1.1.73 | feruloyl esterase A | FAEA is more active than FAEB towards wheat arabinoxylan, whereas FAEB is more active than FAEA towards sugar-beet pectin | Aspergillus niger |
3.1.1.73 | feruloyl esterase B | FAEA is more active than FAEB towards wheat arabinoxylan, whereas FAEB is more active than FAEA towards sugar-beet pectin | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | - |
using methyl caffeate as substrate | Aspergillus niger |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.73 | Aspergillus niger | calculated from the deduced amino acid sequence | - |
4.89 |