EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.31 | expression in Escherichia coli | Rhodotorula graminis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 0.47 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 0.53 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 0.74 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
1.1.99.31 | 0.78 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 0.79 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
1.1.99.31 | 1.33 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme | Rhodotorula graminis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.31 | Rhodotorula graminis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.31 | recombinant enzyme | Rhodotorula graminis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.31 | (S)-mandelate + 2 ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+ | - |
? | |
1.1.99.31 | (S)-mandelate + cytochrome c | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + ? | - |
? | |
1.1.99.31 | DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + ferrocyanide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.31 | L-MDH | - |
Rhodotorula graminis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 88 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 92 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 99 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
1.1.99.31 | 155 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 225 | - |
cytochrome c | pH 7.5, 25°C | Rhodotorula graminis | |
1.1.99.31 | 300 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme | Rhodotorula graminis | |
1.1.99.31 | 316 | - |
DL-[2-1H]-mandelate | pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain | Rhodotorula graminis | |
1.1.99.31 | 550 | - |
ferricyanide | pH 7.5, 25°C | Rhodotorula graminis |