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Literature summary extracted from

  • Smekal, O.; Reid, G.A.; Chapman, S.K.
    Substrate analogues as probes of the catalytic mechanism of L-mandelate dehydrogenase from Rhodotorula graminis (1994), Biochem. J., 297, 647-652.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.08
-
 3-hydroxy-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.12
-
 3-methoxy-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.12
-
 4-methoxy-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.15
-
 4-chloro-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.16
-
 4-fluoro-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.17
-
 4-methyl-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.24
-
 L-Mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.26
-
 4-bromo-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.35
-
 D,L-Mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.38
-
 4-chloro-D,L-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 0.74
-
 DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Rhodotorula graminis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 3-hydroxy-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(3-hydroxyphenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 3-methoxy-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(3-methoxyphenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 4-bromo-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(4-bromophenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 4-chloro-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(4-chlorophenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 4-fluoro-D,L-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(4-fluorophenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 4-hydroxy-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(4-hydroxyphenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 4-methoxy-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(4-methoxyphenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 4-methyl-DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-(4-methylphenyl)acetate + ferrocyanide + H2O
-
 ?
1.1.99.31 DL-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + ferrocyanide + H2O
-
 ?
1.1.99.31 L-mandelate + ferricyanide
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + ferrocyanide
-
 ?
1.1.99.31 additional information enzyme-catalyzed reaction proceeds via the same transition state for each substrate and indicates that this transition state is relatively non-polar but has an electron-rich centre at the alpha-carbon position Rhodotorula graminis ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.31 68
-
 4-chloro-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 68
-
 3-methoxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 80
-
 4-methyl-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 93
-
 DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 94
-
 D,L-Mandelate pH 7.5, 25°C, cosubstrate: ferricyanid Rhodotorula graminis
1.1.99.31 98
-
 4-fluoro-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 106
-
 4-methoxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 108
-
 4-bromo-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 108
-
 3-hydroxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 114
-
 L-Mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 116
-
 4-chloro-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis
1.1.99.31 146
-
 4-hydroxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide Rhodotorula graminis