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Literature summary extracted from

  • Huet, J.; Looze, Y.; Bartik, K.; Raussens, V.; Wintjens, R.; Boussard, P.
    Structural characterization of the papaya cysteine proteinases at low pH (2006), Biochem. Biophys. Res. Commun., 341, 620-626.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.6 nutrition at low pH, enzyme undergoes conformational transition leading to instability and rapid degradation by pepsin. To be effective in gut after oral administration, enzyme needs to be protected against acid denaturation and degradation Carica papaya
3.4.22.25 nutrition the enzyme needs to be protected from acid denaturation and proteolysis in the gut after oral administration to be effective as cysteine protease Carica papaya
3.4.22.30 nutrition at low pH, enzyme undergoes conformational transition leading to instability and rapid degradation by pepsin. To be effective in gut after oral administration, enzyme needs to be protected against acid denaturation and degradation Carica papaya

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.25 extracellular latex Carica papaya
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.6 Carica papaya
-
-
-
3.4.22.25 Carica papaya
-
-
-
3.4.22.30 Carica papaya
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.25 native enzyme from latex by ion exchange and adsorption chromatography, and gel filtration Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.6 latex
-
 Carica papaya
-
3.4.22.25 latex
-
 Carica papaya
-

Synonyms

EC Number Synonyms Comment Organism
3.4.22.25 glycine endopeptidase
-
 Carica papaya

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.6 1
-
 at low pH, the enzyme undergoes a conformational transition that instantaneously converts their native form into molten globule that are quite unstable and rapidly degraded by pepsin. The denatured state of these proteinase which results from acid treatment is completely irreversible Carica papaya
3.4.22.6 2 3 enzyme undergoes conformational transition that instantaneously converts the native form into a molten globule state and is completely irreversible Carica papaya
3.4.22.25 additional information
-
 the enzyme undergoes a conformational transition at low pH and 37°C, that instantaneously and irreversibly converts the native form into molten globules, which are unstable and rapidly degraded by pepsin Carica papaya
3.4.22.30 2 3 enzyme undergoes conformational transition that instantaneously converts the native form into a molten globule state and is completely irreversible Carica papaya