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Literature summary extracted from

  • Bratkovic, T.; Lunder, M.; Popovic, T.; Kreft, S.; Turk, B.; Strukelj, B.; Urleb, U.
    Affinity selection to papain yields potent peptide inhibitors of cathepsins L, B, H, and K (2005), Biochem. Biophys. Res. Commun., 332, 897-903.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 CNWAAGYNCGGGS-NH2 synthetic cyclic peptide, cyclization through intramolecular disulfide bonding Carica papaya
3.4.22.2 CNWTLGGYKCGGGS-NH2 synthetic cyclic peptide, cyclization through intramolecular disulfide bonding Carica papaya
3.4.22.2 CWEWGGWHCGGSS-OH synthetic cyclic peptide, cyclization through intramolecular disulfide bonding Carica papaya
3.4.22.2 CWSMMGFQCGGGS-NH2 weak inhibition, synthetic cyclic peptide, cyclization through intramolecular disulfide bonding Carica papaya
3.4.22.2 GNWTLGGYKGG weak inhibition, synthetic cyclic peptide, cyclization head-to-tail Carica papaya
3.4.22.2 additional information no inhibition by synthetic cyclic peptide CTSPRLHPCGGGS-NH2, interaction anaylsis of enzyme with diverse synthetic peptides in a phage display assay, overview Carica papaya
3.4.22.16 CNWAAGYNCGGGS-NH2 cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain Homo sapiens
3.4.22.16 CNWTLGGYKCGGGS-NH2 cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain Homo sapiens
3.4.22.16 CWEWGGWHCGGSS-OH cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain Homo sapiens
3.4.22.16 CWSMMGFQCGGGS-NH2 cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-
3.4.22.16 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 commercial preparation
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 additional information interaction anaylsis of enzyme with diverse synthetic peptides in a phage display assay, overview Carica papaya ?
-
 ?
3.4.22.2 Z-Phe-Arg-4-nitroanilide + H2O
-
 Carica papaya Z-Phe-Arg + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 37
-
 assay at Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6.8
-
 assay at Carica papaya

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.2 0.0063
-
 CNWAAGYNCGGGS-NH2 pH 6.8, 37°C Carica papaya
3.4.22.2 0.0071
-
 CWEWGGWHCGGSS-OH pH 6.8, 37°C Carica papaya
3.4.22.2 0.0286
-
 CNWTLGGYKCGGGS-NH2 pH 6.8, 37°C Carica papaya
3.4.22.16 0.0039
-
 CWSMMGFQCGGGS-NH2 pH 6.8, 22°C Homo sapiens
3.4.22.16 0.0049
-
 CWEWGGWHCGGSS-OH pH 6.8, 22°C Homo sapiens
3.4.22.16 0.0051
-
 CNWTLGGYKCGGGS-NH2 pH 6.8, 22°C Homo sapiens
3.4.22.16 0.0179
-
 CNWAAGYNCGGGS-NH2 pH 6.8, 22°C Homo sapiens