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Literature summary extracted from
- Characterization of Aquifex aeolicus RNase E/G (2005), Biochem. Biophys. Res. Commun., 327, 382-392.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.26.12 | gene rng, overexpression of His10-tagged RNase E/G, Rng, in Escherichia coli strain BL21(DE3) | Aquifex aeolicus |
| 3.1.26.12 | overexpression of His10-tagged enzyme in Escherichia coli strain BL21(DE3) | Aquifex aeolicus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.12 | Mg2+ | required | Aquifex aeolicus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.12 | additional information | Aquifex aeolicus | Aquifex aeolicus RNase E/G is able to selectively cleave internucleotide bonds in the 3'-5' direction, and to cut in intercistronic regions of putative tRNA precursors | ? | - |
? | |
| 3.1.26.12 | RNA + H2O | Aquifex aeolicus | the enzyme is required for RNA processing and degradation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.12 | Aquifex aeolicus | - |
gene rng | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.12 | recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by His-trap chromatography with or without cleavage of the His-tag by factor Xa | Aquifex aeolicus |
| 3.1.26.12 | recombinant His10-tagged RNase E/G from Escherichia coli strain BL21(DE3) by metal affinity chromatography, removal of the His-tag by factorXa and dialysis | Aquifex aeolicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.12 | 9SA RNA + H2O | - |
Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | BR13 RNA + H2O | i.e. 5'GGGACAGUAUUUG3', 3' fluorescein-labeled substrate | Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | additional information | the enzyme prefers 5' monophosphorylated RNA substrates compared to nonphosphorylated or 5' triphosphorylated RNA substrates | Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | additional information | Aquifex aeolicus RNase E/G is able to selectively cleave internucleotide bonds in the 3'-5' direction, and to cut in intercistronic regions of putative tRNA precursors | Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | additional information | site-specific RNase E/G cleavage of RNA using 5'-end-labelled substrates, e.g. L1 RNA, RNAI, and 9S RNA, overview. RNase E/G has a temperature-dependent, endoribonucleolytic activity that is dependent on the 5'-phosphorylation status of RNA. The enzyme site-specifically cleaves oligonucleotides and structured RNAs at locations that are partly overlapping or completely different when compared to the positions of Escherichia coli RNase E and RNase G cleavage sites, RNase E/G shows 3'-5' directionality in cleavage site selection, overview, the cleavage site selection of RNase E/G is temperature-dependent, overview | Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | Omp11 RNA + H2O | - |
Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | RNA + H2O | the enzyme is required for RNA processing and degradation | Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | RNA + H2O | cleavage site specificity is not affected by temperature, selective cleavage at the 5' end of internucleotide bonds in 3' to 5' direction, cleavage pattern, overview | Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | RNA I + H2O | - |
Aquifex aeolicus | ? | - |
? | |
| 3.1.26.12 | tRNA precursors + H2O | the enzyme, cuts intercistronic regions of putative tRNA precursors, overview | Aquifex aeolicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.12 | AqaRng | - |
Aquifex aeolicus |
| 3.1.26.12 | RNase E/G | - |
Aquifex aeolicus |
| 3.1.26.12 | Rng | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.12 | 45 | 60 | recombinant enzyme | Aquifex aeolicus |
| 3.1.26.12 | 45 | 75 | assay at, the cleavage site selection of RNase E/G is temperature-dependent, overview | Aquifex aeolicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.12 | 30 | 85 | recombinant enzyme | Aquifex aeolicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.12 | 8 | - |
assay at | Aquifex aeolicus |
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