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Literature summary extracted from

  • Sivy, T.L.; Shirk, M.C.; Fall, R.
    Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis (2002), Biochem. Biophys. Res. Commun., 294, 71-75.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.3.27 the enzyme activity is very labile and only reproducibly detected when suitable protectants are used in the extraction buffer, especially PMSF is highly stabilizing Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 1,10-phenanthroline weak, 20% inhibition at 10 mM Bacillus subtilis
4.2.3.27 Ca2+ inhibitory at higher concentrations Bacillus subtilis
4.2.3.27 Co2+ inhibits at 1 mM Bacillus subtilis
4.2.3.27 EDTA 40% inhibition at 1 mM, 70% inhibition at 10 mM Bacillus subtilis
4.2.3.27 Fe2+ inhibits at 1 mM Bacillus subtilis
4.2.3.27 Mg2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
4.2.3.27 Mn2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
4.2.3.27 additional information no inhibition by EGTA Bacillus subtilis
4.2.3.27 Zn2+ inhibits at 1 mM Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mg2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
4.2.3.27 Mn2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
4.2.3.27 additional information the enzyme requires low levels of divalent cations Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Bacillus subtilis in presence of a divalent cation isoprene + diphosphate
-
 ir
4.2.3.27 dimethylallyl diphosphate Bacillus subtilis 6051 in presence of a divalent cation isoprene + diphosphate
-
 ir
4.2.3.27 additional information Bacillus subtilis the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview ?
-
 ?
4.2.3.27 additional information Bacillus subtilis 6051 the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Bacillus subtilis
-
-
-
4.2.3.27 Bacillus subtilis 6051
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 native enzyme partially by anion exchange chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate in presence of a divalent cation Bacillus subtilis isoprene + diphosphate
-
 ir
4.2.3.27 dimethylallyl diphosphate in presence of a divalent cation Bacillus subtilis 6051 isoprene + diphosphate
-
 ir
4.2.3.27 additional information the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview Bacillus subtilis ?
-
 ?
4.2.3.27 additional information the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview Bacillus subtilis 6051 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 6.2
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.27 additional information
-
 pH profile Bacillus subtilis