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Literature summary extracted from

  • Ternan, N.G.; Hamilton, J.T.; Quinn, J.P.
    Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6 (2000), Arch. Microbiol., 173, 35-41.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.11.1.3 dialysis of cell-free extract for 24 h at 4°C results in a 72% loss of activity Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.11.1.3 3-phosphonopropionic acid 10 mM, 21% inhibition Burkholderia cepacia
3.11.1.3 Aminomethylphosphonic acid 10 mM, 19% inhibition Burkholderia cepacia
3.11.1.3 EDTA Co2+, Ni2+, Mg2+, Zn2+, Fe3+ and Ca2+ recover activity. Cu2+, Mn2+ and Cs+ do not recover activity Burkholderia cepacia
3.11.1.3 Fe2+ 5 mM, 94% inhibition Burkholderia cepacia
3.11.1.3 glyphosate 10 mM, 23% inhibition Burkholderia cepacia
3.11.1.3 Mn2+ 5 mM, 12% inhibition Burkholderia cepacia
3.11.1.3 Phosphonoacetate 10 mM, 30% inhibition Burkholderia cepacia
3.11.1.3 phosphonobutyric acid 10 mM, 14% inhibition Burkholderia cepacia
3.11.1.3 Phosphonoformate 10 mM, 37% inhibition Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.11.1.3 0.72
-
 3-phosphonopyruvate
-
 Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.11.1.3 Ca2+ 5 mM, 1.5fold activation Burkholderia cepacia
3.11.1.3 Co2+ 5 mM, 4.8fold activation Burkholderia cepacia
3.11.1.3 Cu2+ 5 mM, 1.2fold activation Burkholderia cepacia
3.11.1.3 Mg2+ 5 mM, 3.4fold activation Burkholderia cepacia
3.11.1.3 Ni2+ 5 mM, 4.3fold activation Burkholderia cepacia
3.11.1.3 Zn2+ 5 mM, 1.8fold activation Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.11.1.3 232000
-
 non-denaturing PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.11.1.3 3-phosphonopyruvate + H2O Burkholderia cepacia the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction pyruvate + phosphate
-
 ?
3.11.1.3 3-phosphonopyruvate + H2O Burkholderia cepacia Pal6 the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction pyruvate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.11.1.3 Burkholderia cepacia
-
-
-
3.11.1.3 Burkholderia cepacia Pal6
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.11.1.3 -20°C, 10 weeks, 54% loss of activity Burkholderia cepacia
3.11.1.3 20, 50% loss of activity after 3 days, complete loss of activity after 6 days Burkholderia cepacia
3.11.1.3 4°C, 30 days, 38% loss of activity Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.11.1.3 3-phosphonopyruvate + H2O
-
 Burkholderia cepacia pyruvate + phosphate
-
 ?
3.11.1.3 3-phosphonopyruvate + H2O the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction Burkholderia cepacia pyruvate + phosphate
-
 ?
3.11.1.3 3-phosphonopyruvate + H2O
-
 Burkholderia cepacia Pal6 pyruvate + phosphate
-
 ?
3.11.1.3 3-phosphonopyruvate + H2O the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction Burkholderia cepacia Pal6 pyruvate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.11.1.3 50
-
-
 Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.11.1.3 7.5
-
-
 Burkholderia cepacia